LOCUS       sequence-287010-        1637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3489
FEATURES             Location/Qualifiers
     source          1..1637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1469..1493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1501..1542
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atgctgatga gggtcaagac
       61 atgtcccaag tttcagggaa ggaaagcccc cctgtaagcg atactccaga tgagggcgat
      121 gagcccatgc cgatccccga ggacctctcc accacctcgg gaggacagca aagctccaag
      181 agtgacagag tcgtggccag taatgttaaa gtagagactc agagtgatga agagaatggg
      241 cgtgcctgtg aaatgaatgg ggaagaatgt gcggaggatt tacgaatgct tgatgcctcg
      301 ggagagaaaa tgaatggctc ccacagggac caaggcagct cggctttgtc gggagttgga
      361 ggcattcgac ttcctaacgg aaaactaaag tgtgatatct gtgggatcat ttgcatcggg
      421 cccaatgtgc tcatggttca caaaagaagc cacactggag aacggccctt ccagtgcaat
      481 cagtgcgggg cctcattcac ccagaagggc aacctgctcc ggcacatcaa gctgcattcc
      541 ggggagaagc ccttcaaatg ccacctctgc aactacgcct gccgccggag ggacgccctc
      601 actggccacc tgaggacgca ctccgtcatt aaagaagaaa ctaatcacag tgaaatggca
      661 gaagacctgt gcaagatagg atcagagaga tctctcgtgc tggacagact agcaagtaac
      721 gtcgccaaac gtaagagctc tatgcctcag aaatttcttg gggacaaggg cctgtccgac
      781 acgccctacg acagcagcgc cagctacgag aaggagaacg aaatgatgaa gtcccacgtg
      841 atggaccaag ccatcaacaa cgccatcaac tacctggggg ccgagtccct gcgcccgctg
      901 gtgcagacgc ccccgggcgg ttccgaggtg gtcccggtca tcagcccgat gtaccagctg
      961 cacaagccgc tcgcggaggg caccccgcgc tccaaccact cggcccagga cagcgccgtg
     1021 gagaacctgc tgctgctctc caaggccaag ttggtgccct cggagcgcga ggcgtccccg
     1081 agcaacagct gccaagactc cacggacacc gagagcaaca acgaggagca gcgcagcggt
     1141 ctcatctacc tgaccaacca catcgccccg cacgcgcgca acgggctgtc gctcaaggag
     1201 gagcaccgcg cctacgacct gctgcgcgcc gcctccgaga actcgcagga cgcgctccgc
     1261 gtggtcagca ccagcgggga gcagatgaag gtgtacaagt gcgaacactg ccgggtgctc
     1321 ttcctggatc acgtcatgta caccatccac atgggctgcc acggcttccg tgatcctttt
     1381 gagtgcaaca tgtgcggcta ccacagccag gaccggtacg agttctcgtc gcacataacg
     1441 cgaggggagc accgcttcca catgagctac ccaactttct tgtacaaagt ggttgatatc
     1501 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1561 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgactgcaca
     1621 ggtgaagtga gacaatt
//
