LOCUS       sequence-287013-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3490
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(683..707)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             715..756
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcacagag gaaggccaga
       61 gggccaccag ccatgccagg ggtggggcat agccagactc aggccaaagc acggttgctg
      121 ccaggcgctg acaggaagag gagccgcctc agcaggacaa ggcaggaccc gtgggaagaa
      181 agaagctgga gcaaccagag atggagcaga gctacccctg gccctcgagg gaccagggct
      241 gggggcctgg ctcttggcag gagcgaggcc agtcctgaga atgccgcgcg ggagcggagc
      301 cgggtcagga cgctgcgcca ggccttcttg gccttgcagg ctgctctgcc tgccgtgccg
      361 cccgacacca agctctccaa gttggacgtg ctggtgctcg ccgccagcta catagcccac
      421 ctcacccgca cactcggcca cgagttgcct ggccctgcct ggccgccctt cctgcgtgga
      481 ctccgctact tgcaccctct caagaagtgg ccgatgcgat ctcgtctcta tgctggaggc
      541 ctggggtact ccgatcttga ctccaccaca gccagcaccc ccagccaaag aacaagagat
      601 gcagaggtgg ggtcccaagt ccctggagag gcagatgctc tcctttccac cacaccactc
      661 tcaccagctc ttggtgacaa attgccaact ttcttgtaca aagtggttga tatcggtaag
      721 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
      781 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagac tgctaaactt
      841 caaattactg c
//
