LOCUS       sequence-287016-        1283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3491
FEATURES             Location/Qualifiers
     source          1..1283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1115..1139)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1147..1188
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttcg aattcaccat ggccagtagg
       61 gaagatgagc tgaggaactg tgtggtatgt ggggaccaag ccacaggcta ccactttaat
      121 gcgctgactt gtgagggctg caagggtttc ttcaggagaa cagtcagcaa aagcattggt
      181 cccacctgcc cctttgctgg aagctgtgaa gtcagcaaga ctcagaggcg ccactgccca
      241 gcctgcaggt tgcagaagtg cttagatgct ggcatgagga aagacatgat actgtcggca
      301 gaagccctgg cattgcggcg agcaaagcag gcccagcggc gggcacagca aacacctgtg
      361 caactgagta aggagcaaga agagctgatc cggacactcc tgggggccca cacccgccac
      421 atgggcacca tgtttgaaca gtttgtgcag tttaggcctc cagctcatct gttcatccat
      481 caccagccct tgcccaccct ggcccctgtg ctgcctctgg tcacacactt cgcagacatc
      541 aacactttca tggtactgca agtcatcaag tttactaagg acctgcctgt cttccgttcc
      601 ctgcccattg aagaccagat ctcccttctc aagggagcag ctgtggaaat ctgtcacatc
      661 gtactcaata ccactttctg tctccaaaca caaaacttcc tctgcgggcc tcttcgctac
      721 acaattgaag atggagcccg tgtatctccc acagtggggt tccaggtaga gtttttggag
      781 ttgctctttc acttccatgg aacactacga aaactgcagc tccaagagcc tgagtatgtg
      841 ctcttggctg ccatggccct cttctctcct gaccgacctg gagttaccca gagagatgag
      901 attgatcagc tgcaagagga gatggcactg actctgcaaa gctacatcaa gggccagcag
      961 cgaaggcccc gggatcggtt tctgtatgcg aagttgctag gcctgctggc tgagctccgg
     1021 agcattaatg aggcctacgg gtaccaaatc cagcacatcc agggcctgtc tgccatgatg
     1081 ccgctgctcc aggagatctg cagctgaaag cttgacccag ctttcttgta caaagtggtt
     1141 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1201 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1261 gagttccgcc tcctagatct ggt
//
