LOCUS       sequence-287019-        1943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3492
FEATURES             Location/Qualifiers
     source          1..1943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1775..1799)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1807..1848
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc ttcggtctgg gccagcctct
       61 gggccgtccg tccccactgg ccgggccatg ccgagtcgcc gcgtcgccag accgccggct
      121 gcgccggagc tgggggcctt agggtccccc gacctctcct cactctcgct cgccgtttcc
      181 aggagcacag atgaattgga gatcatcgac gagtacatca aggagaacgg cttcggcctg
      241 gacgggggac agccgggccc gggcgagggg ctgccacgcc tggtgtctcg cggggctgcg
      301 tccctgagca cggtcaccct gggccctgtg gcgcccccag ccacgccgcc gccttggggc
      361 tgccccctgg gccgactagt gtccccagcg ccgggcccgg gcccgcagcc gcacctggtc
      421 atcacggagc agcccaagca gcgcggcatg cgcttccgct acgagtgcga gggccgctcg
      481 gccggcagca tccttgggga gagcagcacc gaggccagca agacgctgcc cgccatcgag
      541 ctccgggatt gtggagggct gcgggaggtg gaggtgactg cctgcctggt gtggaaggac
      601 tggcctcacc gagtccaccc ccacagcctc gtggggaaag actgcaccga cggcatctgc
      661 agggtgcggc tccggcctca cgtcagcccc cggcacagtt ttaacaacct gggcatccag
      721 tgtgtgagga agaaggagat tgaggctgcc attgagcgga agattcaact gggcattgac
      781 ccctacaacg ctgggtccct gaagaaccat caggaagtag acatgaatgt ggtgaggatc
      841 tgcttccagg cctcatatcg ggaccagcag ggacagatgc gccggatgga tcctgtgctt
      901 tccgagcccg tctatgacaa gaaatccaca aacacatcag agctgcggat ttgccgaatt
      961 aacaaggaaa gcgggccgtg caccggtggc gaggagctct acttgctctg cgacaaggtg
     1021 cagaaagagg acatatcagt ggtgttcagc agggcctcct gggaaggtcg ggctgacttc
     1081 tcccaggccg acgtgcaccg ccagattgcc attgtgttca agacgccgcc ctacgaggac
     1141 ctggagattg tcgagcccgt gacagtcaac gtcttcctgc agcggctcac cgatggggtc
     1201 tgcagcgagc cattgccttt cacgtacctg cctcgcgacc atgacagcta cggcgtggac
     1261 aagaagcgga aacgggggat gcccgacgtc cttggggagc tgaacagctc tgacccccat
     1321 ggcatcgaga gcaaacggcg gaagaaaaag ccggccatcc tggaccactt cctgcccaac
     1381 cacggctcag gcccgttcct cccgccgtca gccctgctgc cagaccctga cttcttctct
     1441 ggcaccgtgt ccctgcccgg cctggagccc cctggcgggc ctgacctcct ggacgatggc
     1501 tttgcctacg accctacggc ccccacactc ttcaccatgc tggacctgct gcccccggca
     1561 ccgccacacg ctagcgctgt tgtgtgcagc ggaggtgccg gggccgtggt tggggagacc
     1621 cccggccctg aaccactgac actggactcg taccaggccc cgggccccgg ggatggaggc
     1681 accgccagcc ttgtgggcag caacatgttc cccaatcatt accgcgaggc ggcctttggg
     1741 ggcggcctcc tatccccggg gcctgaagcc acgtacccaa ctttcttgta caaagtggtt
     1801 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1861 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1921 catgcatcca ttaagtatcg tta
//
