LOCUS       sequence-287022-        1937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3493
FEATURES             Location/Qualifiers
     source          1..1937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1769..1793)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1801..1842
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggactcagt ggcttttgag
       61 gatgtgtctg tgagcttcag ccaggaggag tgggctctgc tggctccttc acagaagaaa
      121 ctctacagag atgtgatgca ggaaacattc aagaacctgg catctatagg ggaaaaatgg
      181 gaagacccga atgttgaaga tcaacacaaa aaccaaggac gaaatctaag aagccatacg
      241 ggagagagac tctgtgaagg taaagaaggt agtcaatgtg cagaaaactt cagtcccaat
      301 ctcagtgtga cgaagaagac tgccggagta aaaccatatg agtgtactat ctgtggaaaa
      361 gccttcatgc gtctctcatc ccttactaga cacatgaggt ctcacactgg atacgagcta
      421 tttgagaagc catataaatg taaggagtgt gagaaagcct ttagttatct caaatccttt
      481 caaagacatg aaaggagtca cactggagaa aaaccctata aatgtaaaca atgtggaaaa
      541 accttcatat atcaccagcc ctttcaaaga catgagcgga ctcacattgg agaaaaaccc
      601 tatgaatgta agcaatgtgg aaaagctctt agttgttcca gttcgcttcg agttcatgaa
      661 aggattcaca ctggagaaaa gccctatgaa tgtaaacaat gtgggaaagc cttcagttgt
      721 tccagttcta ttcgagtaca cgaaagaact cacactggag agaaacccta tgcatgtaag
      781 gaatgtggga aagccttcat ttcccacaca agtgttctaa cacacatgat aacacacaac
      841 ggagatagac cttataaatg caaagaatgt ggaaaggcat tcatttttcc cagtttttta
      901 cgagtacatg aaagaattca cactggagag aaaccctata aatgtaaaca atgtggtaaa
      961 gccttcagat gttccacctc cattcaaatt catgaaagaa ttcatactgg agagaagccc
     1021 tataaatgta aagaatgtgg gaaatctttc agtgcacgcc cagcctttcg agtacacgtg
     1081 agagtgcata ctggagagaa accctataag tgtaaagaat gtgggaaagc ctttagtaga
     1141 atcagttact ttcgaataca tgaaaggact cacactggag agaaacccta cgaatgtaaa
     1201 aaatgtggga aaactttcaa ttatcctcta gatttgaaaa tccacaagag aaatcacact
     1261 ggagaaaaac cctatgagtg taaggaatgt gcaaaaacct tcatttctct tgagaacttt
     1321 cgaagacaca tgatcaccca cactggagac ggaccttata aatgtaggga ctgtgggaag
     1381 gtgttcattt ttcctagtgc gttacgaaca catgaaagaa ctcacactgg agagaaaccc
     1441 tatgaatgta aacaatgtgg aaaagccttt agttgttcta gttacattcg gatacataaa
     1501 agaactcaca ctggggagaa accttatgaa tgtaaggaat gcgggaaggc ctttatttat
     1561 cccacaagct ttcaaggaca catgagaatg catactggag agaaacccta taaatgtaaa
     1621 gaatgtggga aggcctttag tcttcacagt tcctttcaaa gacatacaag aattcacaat
     1681 tatgagaaac ctcttgaatg taagcaatgt ggaaaagcct tcagtgtgtc cacatcctta
     1741 aaaaaacata tgagaatgca caatcgattg ccaactttct tgtacaaagt ggttgatatc
     1801 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1861 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatattcaa
     1921 ctataccgac cttggtg
//
