LOCUS       sequence-287025-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3494
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(866..890)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             898..939
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ggagcaaggc tctgccagcg
       61 cccatcccgc tccacccgtc gctgcagctc accaattact ccttcctgca ggccgtgaac
      121 accttcccgg ccacggtgga ccacctgcag ggcctgtacg gtctcagcgc ggtacagacc
      181 atgcacatga accactggac gctggggtat cccaatgtgc acgagatcac ccgctccacc
      241 atcacggaga tggcggcggc gcagggcctc gtggacgcgc gcttcccctt cccggccctg
      301 ccttttacca cccacctatt ccaccccaag cagggggcca ttgcccacgt cctcccagcc
      361 ctgcacaagg accggccccg ttttgacttt gccaatttgg cggtggctgc cacgcaagag
      421 gatccgccta agatgggaga cctgagcaag ctgagcccag gactgggtag ccccatctcg
      481 ggcctcagta aattgactcc ggacagaaag ccctctcgag gaaggttgcc ctccaaaacg
      541 aaaaaagagt ttatctgcaa gttttgcggc agacacttta ccaaatccta caatttgctc
      601 atccatgaga ggacccacac ggacgagagg ccgtacacgt gtgacatctg ccacaaggcc
      661 ttccggaggc aagatcacct gcgggatcac agatacatcc attccaaaga aaaacccttc
      721 aaatgtcagg agtgtgggaa aggattttgt cagtctagaa ctctagcagt tcacaaaact
      781 ttacacatgc agacatcaag ccctacagct gcgagcagtg cggcaaagtg ttcaggcgaa
      841 actgtgatct gcggcggcac agcctgtcca actttcttgt acaaagtggt tgatatcggt
      901 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
      961 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gactacataa
     1021 gctgtagtgc cggc
//
