LOCUS       sequence-287028-        2747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3495
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2579..2603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2611..2652
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgttgtttac cgtaagctgt
       61 agtaaaatga gctcgattgt tgacagagat gacagtagta tttttgatgg gttggtggaa
      121 gaagatgaca aggacaaagc gaaaagagta tctagaaaca aatctgaaaa gaaacgtaga
      181 gatcaattta atgttctcat taaagaactg ggatccatgc ttcctggtaa tgctagaaag
      241 atggacaaat ctactgttct gcagaaaagc attgattttt tacgaaaaca taaagaaatc
      301 actgcacagt cagatgctag tgaaattcga caggactgga aacctacatt ccttagtaat
      361 gaagagttta cacaattaat gttagaggct cttgatggtt tttttttagc aatcatgaca
      421 gatggaagca taatatatgt gtctgagagt gtaacttcat tacttgaaca tttaccatct
      481 gatcttgtgg atcaaagtat atttaatttt atcccagaag gggaacattc agaggtttat
      541 aaaatactct ctactcatct gctggaaagt gattcattaa ccccagaata tttaaaatca
      601 aaaaatcagt tagaattctg ttgtcacatg ctgcgaggaa caatagaccc aaaggagcca
      661 tctacctatg aatatgtaaa atttatagga aatttcaaat ctttaaacag tgtatcctct
      721 tcagcacaca atggttttga aggaactata caacgcacac ataggccatc ttatgaagat
      781 agagtttgtt ttgtagctac tgtcaggtta gctacacctc agttcatcaa ggaaatgtgc
      841 actgttgaag aacccaatga agagtttaca tctagacata gtttagaatg gaagtttctg
      901 tttctagatc acagggcacc acccataata gggtatttgc catttgaagt tctgggaaca
      961 tcaggctatg attactatca tgtggatgac ctagaaaatt tggcaaaatg tcatgagcac
     1021 ttaatgcaat atgggaaagg caaatcatgt tattataggt tcctgactaa ggggcaacag
     1081 tggatttggc ttcagactca ttattatatc acttaccatc agtggaattc aaggccagag
     1141 tttattgttt gtactcacac tgtagtaagt tatgcagaag ttagggctga aagacgacga
     1201 gaacttggca ttgaagagtc tcttcctgag acagctgctg acaaaagcca agattctggg
     1261 tcagataatc gtataaacac agtcagtctc aaggaagcat tggaaaggtt tgatcacagc
     1321 ccaacccctt ctgcctcttc tcggagttca agaaaatcat ctcacacggc cgtctcagac
     1381 ccttcctcaa caccaaccaa gatcccgacg gatacgagca ctccacccag gcagcattta
     1441 ccagctcatg agaagatggt gcaaagaagg tcatcattta gtagtcagtc cataaattcc
     1501 cagtctgttg gttcatcatt aacacagcca gtgatgtctc aagctacaaa tttaccaatt
     1561 ccacaaggca tgtcccagtt tcagttttca gctcaattag gagccatgca acatctgaaa
     1621 gaccaattgg aacaacggac acgcatgata gaagcaaata ttcatcggca acaagaagaa
     1681 ctaagaaaaa ttcaagaaca acttcagatg gtccatggtc aggggctgca gatgtttttg
     1741 caacaatcaa atcctgggtt gaattttggt tccgttcaac tttcttctgg aaattcatct
     1801 aacatccagc aacttgcacc tataaatatg caaggccaag ttgttcctac taaccagatt
     1861 caaagtggaa tgaatactgg acacattggc acaactcagc acatgataca acaacagact
     1921 ttacagagta catcaactca gagtcaacaa aatgtactga gtgggcacag tcagcaaaca
     1981 tctctaccca gtcagacaca gagcactctt acagccccac tgtataacac tatggtgatt
     2041 tctcagcctg cagccggaag catggtccag attccatcta gtatgccaca aaacagcacc
     2101 cagagtgctg cagtaactac attcactcag gacaggcaga taagattttc tcaaggtcaa
     2161 caacttgtga ccaaattagt gactgctcct gtagcttgtg gggcagtcat ggtacctagt
     2221 actatgctta tgggccaggt ggtgactgca tatcctactt ttgctacaca acagcaacag
     2281 tcacagacat tgtcagtaac gcagcagcag cagcagcaga gctcccagga gcagcagctc
     2341 acttcagttc agcaaccatc tcaggctcag ctgacccagc caccgcaaca atttttacag
     2401 acttctaggt tgctccatgg gaatccctca actcaactca ttctctctgc tgcatttcct
     2461 ctacaacaga gcaccttccc tcagtcacat caccagcaac atcagtctca gcaacagcag
     2521 caactcagcc ggcacaggac tgacagcttg cccgaccctt ccaaggttca accacagttg
     2581 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2641 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2701 tatatatctt gtggaaagga cgagtcaacc aaaaggactt gcgatct
//
