LOCUS       sequence-28703-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pQCXIB CMV/TO DsRed2 (w444-1)
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(203..227)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    268..286
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    289..307
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(309..512)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(338..358)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggggccgccc ttggtcacct tcagcttcac ggtgttgtgg ccctcgtagg ggcggccctc
       61 gccctcgccc tcgatctcga actcgtggcc gttcacggtg ccctccatgc gcaccttgaa
      121 gcgcatgaac tcggtgatga cgttctcgga ggaggccatg gtggcgaccg gtggatccag
      181 tcgactgaat tggttccttt aaagcctgct tttttgtaca aacttgttga tatctgcaga
      241 attccaccac actggactag tcgacgatct ctatcactga tagggagatc tctatcactg
      301 atagggagag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
      361 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
      421 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
      481 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
      541 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
      601 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
      661 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatg
//
