LOCUS       sequence-287031-        1970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3496
FEATURES             Location/Qualifiers
     source          1..1970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1802..1826)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1834..1875
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcctccgg tgcgtataac
       61 ccgtatatag agataattga acaacccagg cagaggggaa tgcgttttag atacaaatgt
      121 gaagggcgat cagcaggcag cattccaggg gagcacagca cagacaacaa ccgaacatac
      181 ccttctatcc agattatgaa ctattatgga aaaggaaaag tgagaattac attagtaaca
      241 aagaatgacc catataaacc tcatcctcat gatttagttg gaaaagactg cagagacggc
      301 tactatgaag cagaatttgg acaagaacgc agacctttgt ttttccaaaa tttgggtatt
      361 cgatgtgtga agaaaaaaga agtaaaagaa gctattatta caagaataaa ggcaggaatc
      421 aatccattca atgtccctga aaaacagctg aatgatattg aagattgtga cctcaatgtg
      481 gtgagactgt gttttcaagt ttttctccct gatgaacatg gtaatttgac gactgctctt
      541 cctcctgttg tctcgaaccc aatttatgac aaccgtgctc caaatactgc agaattaagg
      601 atttgtcgtg taaacaagaa ttgtggaagt gtcagaggag gagatgaaat atttctactt
      661 tgtgacaaag ttcagaaaga tgacatagaa gttcgttttg tgttgaacga ttgggaagca
      721 aaaggcatct tttcacaagc tgatgtacac cgtcaagtag ccattgtttt caaaactcca
      781 ccatattgca aagctatcac agaacccgta acagtaaaaa tgcagttgcg gagaccttct
      841 gaccaggaag ttagtgaatc tatggatttt agatatctgc cagatgaaaa agatacttac
      901 ggcaataaag caaagaaaca aaagacaact ctgcttttcc agaaactgtg ccaggatcac
      961 gttaattttc ctgagagacc aagacctggt ctcctcggtt caattggaga aggaagatac
     1021 ttcaaaaaag aaccaaactt gttttctcat gatgcagttg tgagagaaat gcctacaggg
     1081 gtttcaagtc aagcagaatc ctactatccc tcacctgggc ccatctcaag tggattgtca
     1141 catcatgcct caatggcacc tctgccttct tcaagctggt catcagtggc ccaccccacc
     1201 ccacgctcag gcaatacaaa cccactgagt agtttttcaa caaggacact tccttctaat
     1261 tcgcaaggta tcccaccatt cctgagaata cctgttggga atgatttaaa tgcttctaat
     1321 gcttgcattt acaacaatgc cgatgacata gtcggaatgg aagcgtcatc catgccatca
     1381 gcagatttat atggtatttc tgatcccaac atgctgtcta attgttctgt gaatatgatg
     1441 acaaccagca gtgacagcat gggagagact gataatccaa gacttctgag catgaatctt
     1501 gaaaacccct catgtaattc agtgttagac ccaagagact tgagacagct ccatcagatg
     1561 tcctcttcca gtatgtcagc aggcgccaat tccaatacta ctgtttttgt ttcacaatca
     1621 gatgcatttg agggatctga cttcagttgt gcagataaca gcatgataaa tgagtcggga
     1681 ccatcaaaca gtactaatcc aaacagtcat ggttttgttc aagatagtca gtattcaggt
     1741 attggcagta tgcaaaatga gcaattgagt gactcctttc catatgaatt ttttcaagta
     1801 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1861 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1921 ctttatatat cttgtggaaa ggacgacgca ccacacatag actcaagccc
//
