LOCUS       sequence-287034-        2417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3497
FEATURES             Location/Qualifiers
     source          1..2417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    151..162
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(2249..2273)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2281..2322
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atgacgacaa accttttcaa
       61 ccaaaaaaca tttcaaaaat ggcagaactc tttatggaat gtgaagaaga agagctagag
      121 ccatggcaga agaaagtaga agaaactcag gatgaggatg acgatgaact gatctttgtt
      181 ggagagatat caagttcaaa accagccatt tcaaatattt tgaacagagg ccactccagc
      241 tcatcttcaa aaggaataaa gagtgagcca cacagtccag gtattcctga aatattcagg
      301 actgcaagtc aacgctgcag agacccacca tcaaatccag tggctgcctc gcctagattt
      361 catcttgtat ctaaatcttc acaaagctct gttactgttg agaatgcgtc taaacctgat
      421 tttacaaaga attcacaagt tggatcggat aattcttcaa ttttactgtt tgactcgacc
      481 caggaatcac taccaccatc ccaagacata ccagcaattt ttagagaagg tatgaaaaat
      541 acttcatatg tgttgaaaca tccttctact tctaaagtaa acagtgttac tccaaaaaaa
      601 ccaaagacca gtgaagatgt tcctcagata aatccctcca cttcattgcc tttaattggc
      661 tctcctccag tgacatcctc ccaagttatg ctgtcaaaag gtacaaatac ctcatctcca
      721 tatgatgctg gagcagatta cctaagagct tgtccaaagt gcaatgttca gttcaatctt
      781 ttggatcctt tgaaatacca catgaagcat tgttgtccag acatgataac taaatttttg
      841 ggagtaattg ttaaatcaga acgtccatgt gatgaagaca agactgattc agagacagga
      901 aagttgatca tgttagtcaa tgagttttat tatggaaggc atgaaggagt cactgagaaa
      961 gagccaaaga cttacacaac ctttaaatgc ttcagttgct cgaaagttct taaaaataat
     1021 attaggttta tgaaccacat gaaacatcac ttggaacttg agaagcagaa caatgaaagc
     1081 tgggaaaacc acaccacctg ccagcactgt taccggcaat atcccacacc cttccaactg
     1141 cagtgccaca ttgagagtac acacactccc catgagtttt ctactatttg caaaatctgt
     1201 gaattatcat ttgaaacaga gcatattctt ttacaacata tgaaggacac ccataaacct
     1261 ggtgaaatgc catatgtttg ccaggtttgc cagtttagat catcaacatt ttctgatgta
     1321 gaagctcatt ttagagcagc ccatgaaaac actaagaact tgctatgtcc attttgcctc
     1381 aaagttagta aaatggcaac cccctacatg aatcattaca tgaagcatca gaaaaaagga
     1441 gttcatcgtt gcccaaaatg cagactacaa tttttgacca gcaaggagaa agctgaacat
     1501 aaggcgcagc atcgtacatt tataaagcct aaagaactag aaggattgcc tcctggagca
     1561 aaagttacta ttcgagcttc acttggacct ctccagtcaa aattaccaac tgcacctttc
     1621 ggttgcgctc caggcacttc ttttcttcag gtcacacctc cgacatctca aaatacaact
     1681 gctagaaatc ctagaaaatc taatgccagt agatctaaga caagtaagct tcatgcaact
     1741 acatccactg caagtaaagt taatacaagt aagccaaggg gacgtatagc taagtccaaa
     1801 gcaaaaccct cttacaagca aaagcgacag cgcaacagaa aaaataaaat gagccttgct
     1861 ttgaagaaca taaggtgtcg tcggggcatt cacaagtgca ttgaatgtca ttccaaaata
     1921 aaagattttg caagccactt ttctatatac atccactgca gtttttgcaa gtacaatact
     1981 aactgtaaca aagcctttgt aaatcatatg atgagctctc atagcaacca tccaggtaaa
     2041 cggttttgta ttttcaagaa gcattcagga actctcaggg gcattactct agtgtgcctt
     2101 aaatgtgatt tcctagctga ttcttccggc ttagatcgta tggctaaaca cttaagtcaa
     2161 cgtaaaactc atacttgcca agttataata gagaatgttt ccaaaagtac ctcaacttct
     2221 gaacccacta ctggctgctc attgaaatac ccaactttct tgtacaaagt ggttgatatc
     2281 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     2341 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatagaaag
     2401 acccttcctt ctaccca
//
