LOCUS       sequence-287037-        1160 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3498
FEATURES             Location/Qualifiers
     source          1..1160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(992..1016)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1024..1065
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cgaagcgtgg gaaaaaggga
       61 gcggtggcgg aagacgggga tgagctcagg acagagccag aggccaagaa gagtaagacg
      121 gccgcaaaga aaaatgacaa agaggcagca ggagagggcc cagccctgta tgaggacccc
      181 ccagatcaga aaacctcacc cagtggcaaa cctgccacac tcaagatctg ctcttggaat
      241 gtggatgggc ttcgagcctg gattaagaag aaaggattag attgggtaaa ggaagaagcc
      301 ccagatatac tgtgccttca agagaccaaa tgttcagaga acaaactacc agctgaactt
      361 caggagctgc ctggactctc tcatcaatac tggtcagctc cttcggacaa ggaagggtac
      421 agtggcgtgg gcctgctttc ccgccagtgc ccactcaaag tttcttacgg cataggcgat
      481 gaggagcatg atcaggaagg ccgggtgatt gtggctgaat ttgactcgtt tgtgctggta
      541 acagcatatg tacctaatgc aggccgaggt ctggtacgac tggagtaccg gcagcgctgg
      601 gatgaagcct ttcgcaagtt cctgaagggc ctggcttccc gaaagcccct tgtgctgtgt
      661 ggagacctca atgtggcaca tgaagaaatt gaccttcgca accccaaggg gaacaaaaag
      721 aatgctggct tcacgccaca agagcgccaa ggcttcgggg aattactgca ggctgtgcca
      781 ctggctgaca gctttaggca cctctacccc aacacaccct atgcctacac cttttggact
      841 tatatgatga atgctcgatc caagaatgtt ggttggcgcc ttgattactt tttgttgtcc
      901 cactctctgt tacctgcatt gtgtgacagc aagatccgtt ccaaggccct cggcagtgat
      961 cactgtccta tcaccctata cctagcactg tgcccaactt tcttgtacaa agtggttgat
     1021 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1081 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatcta
     1141 tgagacgtca tcgcggcctg
//
