LOCUS       sequence-287040-        1592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3499
FEATURES             Location/Qualifiers
     source          1..1592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1424..1448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1456..1497
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccagcaacag cagctcctgc
       61 ccgacacctg ggggcgggca cctcaatggg tacccggtgc ctccctacgc cttcttcttc
      121 ccccctatgc tgggtggact ctccccgcca ggcgctctga ccactctcca gcaccagctt
      181 ccagttagtg gatatagcac accatcccca gccaccattg agacccagag cagcagttct
      241 gaagagatag tgcccagccc tccctcgcca ccccctctac cccgcatcta caagccttgc
      301 tttgtctgtc aggacaagtc ctcaggctac cactatgggg tcagcgcctg tgagggctgc
      361 aagggcttct tccgccgcag catccagaag aacatggtgt acacgtgtca ccgggacaag
      421 aactgcatca tcaacaaggt gacccggaac cgctgccagt actgccgact gcagaagtgc
      481 tttgaagtgg gcatgtccaa ggagtctgtg agaaacgacc gaaacaagaa gaagaaggag
      541 gtgcccaagc ccgagtgctc tgagagctac acgctgacgc cggaggtggg ggagctcatt
      601 gagaaggtgc gcaaagcgca ccaggaaacc ttccctgccc tctgccagct gggcaaatac
      661 actacgaaca acagctcaga acaacgtgtc tctctggaca ttgacctctg ggacaagttc
      721 agtgaactct ccaccaagtg catcattaag actgtggagt tcgccaagca gctgcccggc
      781 ttcaccaccc tcaccatcgc cgaccagatc accctcctca aggctgcctg cctggacatc
      841 ctgatcctgc ggatctgcac gcggtacacg cccgagcagg acaccatgac cttctcggac
      901 gggctgaccc tgaaccggac ccagatgcac aacgctggct tcggccccct caccgacctg
      961 gtctttgcct tcgccaacca gctgctgccc ctggagatgg atgatgcgga gacggggctg
     1021 ctcagcgcca tctgcctcat ctgcggagac cgccaggacc tggagcagcc ggaccgggtg
     1081 gacatgctgc aggagccgct gctggaggcg ctaaaggtct acgtgcggaa gcggaggccc
     1141 agccgccccc acatgttccc caagatgcta atgaagatta ctgacctgcg aagcatcagc
     1201 gccaaggggg ctgagcgggt gatcacgctg aagatggaga tcccgggctc catgccgcct
     1261 ctcatccagg aaatgttgga gaactcagag ggcctggaca ctctgagcgg acagccgggg
     1321 ggtggggggc gggacggggg tggcctggcc cccccgccag gcagctgtag ccccagcctc
     1381 agccccagct ccaacagaag cagcccggcc acccactccc cgtgcccaac tttcttgtac
     1441 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1501 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1561 aaggacgatt tggtgcccct tcgcagccga cc
//
