LOCUS       sequence-287049-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3502
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(815..839)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             847..888
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcgcctctc ctcctcccca
       61 cctcgtggcc cgcagcagct ctccagcttt ggctccgtgg actggctctc ccagagcagc
      121 tgctcagggc cgacccacac ccccaggcct gccgacttct ccctggggag cctccctggc
      181 ccgggccaga catccggcgc cagggagccc cctcaggccg tcagcatcaa ggaggccgcc
      241 gggtcctcaa atctgcctgc gccggagagg accatggccg ggttgagtaa ggagccaaat
      301 accttgcggg ccccccgtgt ccgcacagcc ttcaccatgg agcaggtccg caccttggag
      361 ggcgtcttcc agcaccacca gtacctgagc cctctggagc ggaagaggct ggccagggag
      421 atgcagctct cagaggtcca gataaaaacc tggtttcaga atcgccgcat gaaacacaaa
      481 cggcaaatgc aggaccccca gctgcacagc cccttctcgg ggtctctcca tgcgccccca
      541 gctttctact caacgtcttc tggccttgcc aatggcctgc agctgctgtg cccttgggca
      601 cccctgtccg ggccccaggc tctgatgctg ccccctggct ccttctgggg tctctgccaa
      661 gtggcacaag aggccctggc atctgcggga gcttcctgct gcgggcagcc tctggcgtcc
      721 caccccccta ccccaggccg gccttcgctg ggaccagccc tgtccacggg gccccggggc
      781 ctgtgtgcta tgccacagac gggggatgca tttttgccaa ctttcttgta caaagtggtt
      841 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
      901 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
      961 cacaacacct cttctcgcca acg
//
