LOCUS       sequence-287052-        1244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3503
FEATURES             Location/Qualifiers
     source          1..1244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1076..1100)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1108..1149
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctctggacgt gaagtctcgg
       61 gcaaagcgtt atgagaagct ggacttcctt ggggagggac agtttgccac cgtttacaag
      121 gccagagata agaacaccaa ccaaattgtc gccattaaga aaatcaaact tggacataga
      181 tcagaagcta aagatggtat aaatagaacc gccttaagag agataaaatt attacaggag
      241 ctaagtcatc caaatataat tggtctcctt gatgcttttg gacataaatc taatattagc
      301 cttgtctttg attttatgga aactgatcta gaggttataa taaaggataa tagtcttgtg
      361 ctgacaccat cacacatcaa agcctacatg ttgatgactc ttcaaggatt agaatattta
      421 catcaacatt ggatcctaca tagggatctg aaaccaaaca acttgttgct agatgaaaat
      481 ggagttctaa aactggcaga ttttggcctg gccaaatctt ttgggagccc caatagagct
      541 tatacacatc aggttgtaac caggtggtat cgggcccccg agttactatt tggagctagg
      601 atgtatggtg taggtgtgga catgtgggct gttggctgta tattagcaga gttacttcta
      661 agggttcctt ttttgccagg agattcagac cttgatcagc taacaagaat atttgaaact
      721 ttgggcacac caactgagga acagtggccg gacatgtgta gtcttccaga ttatgtgaca
      781 tttaagagtt tccctggaat acctttgcat cacatcttca gtgcagcagg agacgactta
      841 ctagatctca tacaaggctt attcttattt aatccatgtg ctcgaattac ggccacacag
      901 gcactgaaaa tgaagtattt cagtaatcgg ccagggccaa cacctggatg tcagctgcca
      961 agaccaaact gtccagtgga aaccttaaag gagcaatcaa atccagcttt ggcaataaaa
     1021 aggaaaagaa cagaggcctt agaacaagga ggattgccca agaaactaat tttttaccca
     1081 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1141 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1201 atatcttgtg gaaaggacga tgcgactaga ctgatggcac gatt
//
