LOCUS       sequence-287055-        2009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3504
FEATURES             Location/Qualifiers
     source          1..2009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1841..1865)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1873..1914
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcagcctc ggcgcaggtg
       61 tctgtgacct ttgaggatgt ggctgtgaca ttcacccagg aggagtgggg acagttggat
      121 gcagcccaga gaaccttgta tcaggaggtg atgctggaga cctgcggact tctcatgtct
      181 ctgggctgtc ctttgttcaa accagagctg atctaccagt tggatcacag acaggagcta
      241 tggatggcta caaaagacct ctcccaaagc tcctatccag gtgacaacac aaaacccaag
      301 accacagagc ctaccttttc tcacctggcc ttgcctgagg aagtcttact ccaggaacaa
      361 ctgacacaag gagcctcaaa gaactcccaa ttagggcaat ccaaggatca ggatgggcca
      421 tctgaaatgc aagaagtcca cttgaaaata gggataggcc cccagcgggg gaagctgctg
      481 gagaaaatga gttctgaacg tgatggtttg gggtcagatg atggtgtatg tacaaagatt
      541 acacagaaac aagtttcaac agaaggtgat ctctatgaat gtgattcaca tggaccagtt
      601 acagatgcct tgattcgcga agagaaaaat tcctataaat gtgaggaatg cgggaaagtg
      661 tttaaaaaga atgccctcct tgttcagcat gaacggattc acactcaagt gaagccctat
      721 gaatgcacag agtgtgggaa aacctttagc aagagcactc atcttcttca gcacctcatc
      781 atccacactg gggagaagcc ctataagtgc atggagtgtg ggaaggcttt taaccgcagg
      841 tcacacctca cacggcacca gcggattcac agtggagaga agccttataa gtgcagtgaa
      901 tgtggaaagg ccttcaccca ccgctccact tttgtcttgc atcacaggag ccacactgga
      961 gaaaaaccct ttgtgtgcaa agagtgtggc aaagcctttc gagataggcc aggtttcatt
     1021 cgacactaca tcatccacac gggagagaag ccctatgagt gcattgagtg tgggaaggcc
     1081 ttcaaccgcc ggtcatacct cacgtggcac caacagattc acactggagt gaaacccttt
     1141 gaatgcaacg agtgtggaaa agctttttgc gagagtgcag acctcattca acactacatt
     1201 atccacactg gggagaagcc ctataagtgc atggagtgtg ggaaggcgtt caaccgtagg
     1261 tcacacctca agcagcatca acggattcac actggggaga agccttatga atgcagtgaa
     1321 tgtggaaagg ccttcaccca ctgctccact tttgtcttgc ataaaaggac ccacacagga
     1381 gaaaaaccct atgaatgcaa agaatgtgga aaagccttta gtgatagggc agacctcatt
     1441 cgccacttca gcatccacac tggagagaaa ccctatgagt gcgtggagtg tggaaaggcc
     1501 ttcaaccgca gctcacacct cacgaggcac caacagattc acactggaga gaaaccctat
     1561 gaatgcatcc agtgtgggaa agccttttgc cggagcgcaa accttattcg acactccatc
     1621 attcacactg gagagaagcc gtatgaatgc agtgagtgtg gaaaggcttt taatcgcggc
     1681 tcatccctca cacatcatca aaggattcat actgggagaa accctaccat tgtaacagat
     1741 gtgggaagac cttttatgac tgcacagact tcagtcaaca tccaggaact tttattaggg
     1801 aaagagtttt tgaatatcac cactgaagaa aatctgtggt tgccaacttt cttgtacaaa
     1861 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1921 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1981 gacgaggcct tagcgaagag gtgacagac
//
