LOCUS       sequence-287061-        1424 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3506
FEATURES             Location/Qualifiers
     source          1..1424
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1256..1280)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1288..1329
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc agcgaatgat ccaacaattt
       61 gctgctgaat atacctcaaa aaatagctct actcaggacc ccagccagcc caatagcaca
      121 aagaaccaaa gcctgccgaa agcatctcca gtcaccacct ctcccacggc tgcaactact
      181 cagaaccctg tgctcagcaa acttctcatg gctgaccaag actcacctct ggaccttact
      241 gtcagaaagt ctcagtcaga acctagcgaa caagacggtg tacttgatct gtccactaag
      301 aaaagtccat gtgctggcag cacttccctg agccactctc caggctgctc cagtactcaa
      361 gggaacgggc gacctgggag acccagccag taccgcccag acggacttcg gagtggtgat
      421 ggggtacctc caagaagctt acaggatgga accagggaag gttttggaca ctccacatca
      481 ctcaaagttc cactggctcg atccctgcag attagtgaag aactactgag cagaaaccaa
      541 ttgtccacag ctgccagcct tgggccatct ggattacaga atcatggaca acacttaata
      601 ttatccaggg aagcctcttg ggcaaaacca cattacgagt tcaacctcag ccgtatgaag
      661 ttcaggggaa atggtgcact cagcaacatc agtgaccttc cttttcttgc agaaaactct
      721 gcctttccaa aaatggcact tcaagcaaaa caagatggaa aaaaggatgt gagccattca
      781 tctcctgtag atttaaagat accacaagtt cgaggaatgg atctttcttg ggagtctcgc
      841 actggtgatc agtacagcta tagctctttg gtaatgggtt cacaaacgga gagcgcgctt
      901 agtaaaaaat taagggctat tcttccaaaa caaagtagaa aaagcatgtt agatgctgga
      961 cccgattctt ggggctcaga tgctgagcag tctacctctg gacagccata tcccacatcg
     1021 gatcaagaag gagaccctgg ctccaagcag cctcggaaga aaagagggcg ttacagacag
     1081 tacaacagtg agatactgga ggaagcaatc tcagtggtta tgagtggaaa aatgagtgtt
     1141 tccaaagctc agagtattta tgggattccc cacagtacac tggagtacaa agtaaaggag
     1201 aggctgggca ctttgaaaaa ccctccaaag aaaaagatga aattaatgag tggatgccca
     1261 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1321 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1381 atatcttgtg gaaaggacga gcatggccag gcaatccaat atcg
//
