LOCUS       sequence-287064-        1502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3507
FEATURES             Location/Qualifiers
     source          1..1502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1255..1266)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1334..1358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1366..1407
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtttcatag tccgcggcgg
       61 ctctgctcgg ccctgctgca gagggacgcg cccggcctgc gccgcctgcc cgccccaggg
      121 ctgcgccgcc cgttgtcccc gccggctgct gttcccaggc ccgcatcccc ccggctgctg
      181 gcggcggcct cggcggcctc gggcgccgcg aggtcgtgtt cccgaacagt gtgttccatg
      241 ggaaccggta caagcagact ctatagtgct ctcgccaaga cactgaacag cagcgctgcc
      301 tcccagcacc cagagtattt ggtgtcacct gacccagagc atctggagcc cattgatcct
      361 aaagagcttc ttgaggaatg cagggccgtc ctgcacaccc gacctccccg gttccagagg
      421 gattttgtgg atctgaggac agattgccct agtacccacc cacctatcag ggttatgcaa
      481 tggaacatcc tcgcccaagc tcttggagaa ggcaaagaca actttgtaca gtgccctgtt
      541 gaagcactca aatgggaaga aaggaaatgt ctcatcctgg aagaaatcct ggcctaccag
      601 cctgatatat tgtgcctcca agaggtggac cactattttg acaccttcca gccactcctc
      661 agtagactag gctatcaagg cacgtttttc cccaaaccct ggtcaccttg tctagatgta
      721 gaacacaaca atggaccaga tggttgtgcc ttattttttc ttcaaaaccg attcaagcta
      781 gtcaacagtg ccaatattag gctgacagcc atgacattga aaaccaacca ggtggccatt
      841 gcacagaccc tggagtgcaa ggagtcaggc cgacagttct gcatcgctgt tacccatcta
      901 aaagcacgca ctggctggga gcggtttcga tcagctcaag gctgtgacct ccttcagaac
      961 ctgcaaaaca tcacccaagg agccaagatt ccccttattg tgtgtgggga cttcaatgca
     1021 gagccaacag aagaggtcta caaacacttt gcttcctcca gcctcaacct gaacagcgcc
     1081 tacaagctgc tgagtgctga tgggcagtca gaacccccat acactacctg gaagatccgg
     1141 acctcagggg agtgcaggca caccctggat tacatctggt attctaaaca tgctctaaat
     1201 gtaaggtcag ctctcgatct gctcactgaa gaacagattg gacccaacag gttaccttcc
     1261 ttcaattatc cttcagacca cctgtctcta gtgtgtgact tcagctttac tgaggaatct
     1321 gatggacttt cattgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1381 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1441 tcgatttctt ggctttatat atcttgtgga aaggacgaat ctgccgaggg cgagatcctc
     1501 tt
//
