LOCUS       sequence-287067-        1490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3508
FEATURES             Location/Qualifiers
     source          1..1490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1322..1346)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1354..1395
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgactctagt cacagcagga
       61 ggggcttgga caggccctgg ttgttggcat gaagtgaagg atgaagagtc atcttctgaa
      121 cagagcattt ctatagcagt gtcacatgtt aatacttcca aggcaggttt gcccgcacag
      181 acggctctcc cttgtgacat atgtggcccc atcttgaaag atattttgca cctggatgaa
      241 caccagggta cacaccatgg actgaaactt cacacatgtg gggcatgtgg gagacaattc
      301 tggttcagtg caaaccttca tcagtaccag aagtgttaca gtatagagca acccttaaga
      361 agggataaaa gtgaggcctc aattgtgaag aactgcacag ttagcaaaga acctcatccg
      421 tcagagaagc cctttacgtg taaggaggag cagaaaaact tccaggctac tttgggtggc
      481 tgccaacaaa aggccatcca cagtaagagg aagacacaca ggagcactga gagtggggat
      541 gcatttcatg gtgaacaaat gcattacaag tgcagtgaat gtgggaaagc tttcagccgc
      601 aaagacacac ttgtccagca ccagagaatt catagtggag agaagcctta tgagtgcagc
      661 gaatgtggga aagccttcag ccgcaaagct acacttgtcc agcatcagag aatccatact
      721 ggagaaaggc cttatgaatg cagcgaatgt ggaaaaacct tcagtcgaaa agacaacctt
      781 actcagcaca agagaatcca cactggagaa atgccttata agtgcaatga atgtgggaaa
      841 tattttagcc atcactccaa tctaattgta caccagagag ttcacaatgg agcaaggcct
      901 tataagtgca gtgattgtgg gaaagtcttc agacacaaat ctacacttgt tcagcatgag
      961 agtattcaca ctggagaaaa tccttatgat tgcagtgatt gtgggaaatc ctttggccac
     1021 aaatacaccc tcattaaaca tcagcgaatt cacactgagt caaagccgtt tgagtgcatt
     1081 gaatgcggga aattctttag tcgaagttct gactatattg cacaccagag ggttcacact
     1141 ggtgaaaggc cttttgtgtg cagtaaatgt gggaaagact ttatcagaac ctcccacctt
     1201 gttcgacacc aaagagttca cactggagaa aggccatatg agtgcagtga atgtgggaag
     1261 gcctacagct taagctccca cctcaatcgg caccagaaag ttcacactgc aggcaggctt
     1321 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1381 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1441 ctttatatat cttgtggaaa ggacgagccg tgatggttta tgcccatgtt
//
