LOCUS       sequence-287070-        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3509
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1817..1841)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1849..1890
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atagaatgac agaagatgct
       61 cttcgcttga atctgttgaa gcggagcttg gacccagcag atgagcgaga tgatgtcctg
      121 gcaaagcgac tcaaaatgga ggggcatgag gccatggaac gtctgaaaat gttggcattg
      181 ctcaaaagga aggatttggc aaatcttgag gtgccacatg agttacccac caaacaggat
      241 ggcagtggtg tcaagggcta tgaagaaaaa cttaacggga atctcaggcc tcatggagac
      301 aacaggactg ctggaaggcc aggcaaagaa aacatcaatg atgagcctgt ggatatgagt
      361 gctagacgga gtgagccaga gcgaggaagg ctaactccct caccagacat cattgttttg
      421 tctgacaatg aggcttccag tccccgttcc agttccagaa tggaagaaag actcaaagca
      481 gccaacttag agatgtttaa ggggaaaggc attgaggagc ggcagcagct tatcaagcag
      541 ctgagggatg agctacgatt ggaagaagcc cgactggtcc tgttaaagaa actgagacag
      601 agtcagctac agaaagagaa tgtggtccag aagactccag ttgtacagaa tgcagcatct
      661 attgttcagc catctcctgc ccatgtggga cagcagggcc tatctaagct tccctctcgg
      721 cctggggccc aaggggttga acctcaaaat ttgagaacat tacagggtca cagtgtcatc
      781 cgttcagcta ccaataccac ccttccacac atgttgatgt ctcaacgtgt tattgcacca
      841 aacccagccc agctacaggg tcagcggggc ccgcctaagc ctggccttgt acgcaccaca
      901 acacccaaca tgaatcccgc catcaattat caaccgcagt caagttcttc tgttccatgt
      961 cagcgtacaa catcctctgc catctatatg aaccttgctt ctcatatcca gccagggacg
     1021 gtgaacagag tgtcctcgcc acttcctagc cccagcgcca tgactgatgc tgccaactca
     1081 caggctgcag ccaaattggc tcttcgcaaa cagctggaaa agacactcct ggagatccca
     1141 ccccctaaac ctcctgctcc cttacttcat ttcttgccta gtgcagccaa tagcgagttc
     1201 atctacatgg taggcttgga agaagtcgta cagagtgtca ttgacagcca aggcaaaagc
     1261 tgtgcctcac ttctgcgggt tgaacccttt gtatgtgccc agtgccgcac agatttcacc
     1321 cctcactgga agcaagaaaa gaatggtaag attctatgtg agcagtgtat gacctccaac
     1381 cagaaaaagg ctctaaaagc tgaacacacc aaccggctga aaaatgcatt tgtgaaagcc
     1441 ctacagcagg aacaggaaat tgaacagcga ttacagcagc aggcagccct ctcccccact
     1501 acggctccag ctgtgtccag tgtcagtaaa caagagacca tcatgagaca tcatacgctt
     1561 cggcaggctc cacagcccca gagcagcctc cagcgtggca tacccacatc tgcccgctcc
     1621 atgctttcaa actttgcaca ggcaccccag ttgtctgtgc caggtggcct ccttggtatg
     1681 ccaggtgtca acattgcata cttgaatact ggcatcggag gacacaaagg ccccagtttg
     1741 gcagaccgac agcgtgaata ccttttagac atgatccctc cccggtctat atcgcagtcc
     1801 atcagtggac agaaataccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1861 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1921 atttcgattt cttggcttta tatatcttgt ggaaaggacg actctttctg gggtgagcag
     1981 catgt
//
