LOCUS       sequence-287073-        2234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3510
FEATURES             Location/Qualifiers
     source          1..2234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2066..2090)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2098..2139
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cgcgccgggc cctccggagg
       61 ctgagggggg aacagcgcgg ccaggagccc ctcgggcccg gcgccttgca tttcgatctc
      121 cgtgatgacg atgacgcgga agaagaaggg cccaagcggg agcttggtgt ccggcgtccc
      181 gggggcgcag ggaaggaggg cgtccgagtc aacaaccgct tcgagctgat aaacattgac
      241 gatcttgagg atgaccctgt ggtgaacggg gagaggtctg gctgtgcgct cacagacgct
      301 gtggcaccag ggaacaaagg aaggggtcag cgtggaaaca cagagagcaa gacggatgga
      361 gatgacaccg agacagtgcc ctcagagcag tctcatgcaa gtggcaaact ccggaagaag
      421 aaaaaaaaac agaaaaacaa gaaaagcagc acgggagaag catcggaaaa cggactagaa
      481 gatatcgatc gcatcctaga gaggattgag gacagcactg ggttgaaccg tcccggccca
      541 gctcccctga gctccaggaa gcacgttctc tacgtggagc acagacactt gaatccagac
      601 acagaactga aaaggtattt tggtgcccgg gcaatcctgg gggagcaaag gccacggcag
      661 agacaacgtg tgtaccccaa gtgcacatgg ctgaccaccc ctaaaagcac ctggccccgc
      721 tacagcaaac caggtctgtc catgcggctg ctggaatcaa aaaaaggcct ctccttcttt
      781 gcgtttgagc acagtgagga gtaccagcag gctcagcaca agttcctggt ggccgtggag
      841 tctatggagc cgaacaacat cgtggttctg ctccagacga gcccttacca cgttgactca
      901 ctcctgcagc tcagcgatgc ctgccgcttt caagaggatc aggagatggc tcgagacctc
      961 gtagagagag cgctgtacag catggaatgt gcgttccacc ccctgttcag tctcaccagt
     1021 ggggcctgcc ggctggatta ccgcagaccc gagaacagga gcttctacct ggccctctac
     1081 aagcagatga gcttcctgga gaagcgaggc tgcccgcgca cggcgctgga gtactgcaag
     1141 ctcatcctga gtctcgagcc ggatgaggac cccctctgca tgctgctgct catcgaccac
     1201 ctggccttgc gggcccggaa ctacgagtac ctgatccgcc tcttccagga gtgggaggct
     1261 catcggaacc tgtcccagct ccctaatttt gccttctctg ttccactggc gtatttcctg
     1321 ctgagccagc agacagacct ccctgagtgt gagcagagct ctgccaggca gaaggcctct
     1381 ctcctgatac agcaggcgct caccatgttc cctggagtcc tcctgcccct gctcgagtct
     1441 tgcagtgtgc ggcccgacgc cagcgtttcc agtcaccgct tctttggacc caatgctgaa
     1501 ataagccagc cccctgccct gagccagctg gtgaacctgt accttgggag gtcacacttt
     1561 ctctggaaag agcccgccac catgagctgg ctggaggaga acgtccacga ggttctgcaa
     1621 gcagtggacg ccggggaccc agccgtggaa gcctgtgaga accggcggaa ggtgctctac
     1681 cagcgtgcac ccaggaatat ccaccgccat gtgatcctct ctgagatcaa ggaagccgtc
     1741 gctgccctgc ccccggacgt gaccacgcag tctgtgatgg ggtttgatcc tctgcctcct
     1801 tcggacacaa tctactccta cgtcaggcca gagaggctaa gtcctatcag ccatggaaac
     1861 accattgctc tcttcttccg gtcactgttg ccaaactata ccatggaggg ggagaggccc
     1921 gaggaaggag tggctggggg tctgaaccgc aaccagggcc tgaacaggct gatgctggct
     1981 gtgcgcgaca tgatggccaa cttccacctc aacgacctgg aggcaccgca cgaggacgac
     2041 gctgaggggg agggggagtg ggactgccca actttcttgt acaaagtggt tgatatcggt
     2101 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     2161 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga actatagata
     2221 ctgaggttac atct
//
