LOCUS       sequence-287079-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3512
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(761..785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             793..834
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtccaaacc ttcagaccac
       61 atcaagcggc ccatgaacgc cttcatggta tggtcccggg gccagcggcg caagatggcc
      121 caggaaaacc ccaagatgca caactcggag atcagcaaac gcctaggtgc cgaatggaag
      181 cttctgtccg aggcagagaa gcggccatac atcgatgaag ccaagcggct acgcgcccag
      241 cacatgaagg agcaccctga ctacaagtac cgacctcggc gcaagcccaa gaacctgctc
      301 aagaaggaca ggtatgtctt ccccttgccc tacctgggcg acacggaccc gctcaaggcg
      361 gctggcctgc ccgtgggggc ctctgacggc ctcctgagcg cgcccgagaa agcccgggcc
      421 ttcttgccgc cggcctcggc gccctactcc ctgctggacc ccgcgcagtt tagctcgagc
      481 gccatccaga agatgggcga agtgccccac accttggcta ccggcgctct gccctacgcg
      541 tccaccctgg gctaccagaa cggcgccttc ggcagcctca gctgccccag ccagcacacg
      601 cacacgcacc cgtcccccac caaccctggc tacgtggtgc cctgtaactg taccgcctgg
      661 tctgcctcca ccctgcagcc ccccgtcgcc tacatcctct tcccaggcat gaccaagact
      721 ggcatagacc cttattcgtc agcccacgct acggccatgt tgccaacttt cttgtacaaa
      781 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
      841 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      901 gacgatcatt aaaggctggg cttccgaat
//
