LOCUS       sequence-287082-        1424 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3513
FEATURES             Location/Qualifiers
     source          1..1424
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1256..1280)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1288..1329
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc gcggctctgt ggagtgcacc
       61 tggggttggg ggcactgtgc ccccagcccc ctgctccttt ggactctact tctgtttgca
      121 gccccatttg gcctgctggg ggagaagacc cgccaggtgt ctctggaggt catccctaac
      181 tggctgggcc ccctgcagaa cctgcttcat atacgggcag tgggcaccaa ttccacactg
      241 cactatgtgt ggagcagcct ggggcctctg gcagtggtaa tggtggccac caacaccccc
      301 cacagcaccc tgagcgtcaa ctggagcctc ctgctatccc ctgagcccga tgggggcctg
      361 atggtgctcc ctaaggacag cattcagttt tcttctgccc ttgtttttac caggctgctt
      421 gagtttgaca gcaccaacgt gtccgatacg gcagcaaagc ctttgggaag accatatcct
      481 ccatactcct tggccgattt ctcttggaac aacatcactg attcattgga tcctgccacc
      541 ctgagtgcca catttcaagg ccaccccatg aacgacccta ccaggacttt tgccaatggc
      601 agcctggcct tcagggtcca ggccttttcc aggtccagcc gaccagccca accccctcgc
      661 ctcctgcaca cagcagacac ctgtcagcta gaggtggccc tgattggagc ctctccccgg
      721 ggaaaccgtt ccctgtttgg gctggaggta gccacattgg gccagggccc tgactgcccc
      781 tcaatgcagg agcagcactc catcgacgat gaatatgcac cggccgtctt ccagttggac
      841 cagctactgt ggggctccct cccatcaggc tttgcacagt ggcgaccagt ggcttactcc
      901 cagaagccgg ggggccgaga atcagccctg ccctgccaag cttcccctct tcatcctgcc
      961 ttagcatact ctcttcccca gtcacccatt gtccgagcct tctttgggtc ccagaataac
     1021 ttctgtgcct tcaatctgac gttcggggct tccacaggcc ctggctattg ggaccaacac
     1081 tacctcagct ggtcgatgct cctgggtgtg ggcttccctc cagtggacgg cttgtcccca
     1141 ctagtcctgg gcatcatggc agtggccctg ggtgccccag ggctcatgct gctagggggc
     1201 ggcttggttc tgctgctgca ccacaagaag tactcagagt accagtccat aaattaccca
     1261 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1321 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1381 atatcttgtg gaaaggacga acccaagggt cggaagctcc aact
//
