LOCUS       sequence-287085-        1145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3514
FEATURES             Location/Qualifiers
     source          1..1145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(977..1001)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1009..1050
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcggacgg cgacagcggc
       61 agcgagcgcg gcggcggcgg tgggccgtgc gggttccagc ccgcgtcccg cggcggcggc
      121 gagcaagaga cgcaggagct ggcctcgaag cggctggaca tccagaacaa gcgcttctac
      181 ttagatgtga agcagaacgc caagggccgc ttcctcaaga tcgccgaggt gggcgcgggc
      241 ggttccaaga gccgcctcac gctgtccatg gcggtggccg ccgagttccg cgactcgctg
      301 ggcgacttca tagaacacta cgcgcagctg ggccctagca gccccgagca gctggcggcg
      361 ggcgccgagg agggcggcgg gccgcggcgc gcgctcaaga gcgaattctt ggtgcgtgag
      421 aaccgcaagt actacctgga cctcaaggag aaccagcgcg gccgcttcct gcgcatccgc
      481 caaacggtca accgcggcgg tggcggcttc ggcgcgggcc ccgggccggg cggcttgcag
      541 agcggccaga ccatcgcgct gcctgcgcag ggcctcatcg agttccgcga cgcgctggcg
      601 aagctcatag acgactacgg aggcgaggac gacgagctgg caggcggccc gggaggcggc
      661 gccgggggcc cagggggcgg cctgtatgga gagctcccgg agggcacctc catcaccgtg
      721 gactccaagc gcttcttctt cgatgtgggc tgcaacaaat acggggtgtt tctgcgagtg
      781 agcgaggtga agccgtccta ccgcaatgcc atcaccgtac ccttcaaagc ctggggcaag
      841 ttcggaggcg ccttttgccg gtatgcggat gagatgaaag aaatccagga acgacagagg
      901 gataagcttt atgagcgacg tggtgggggc agcggcggcg gcgaagagtc agagggtgag
      961 gaggtggatg aggatttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1021 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1081 atttcgattt cttggcttta tatatcttgt ggaaaggacg agtacttact actcccacca
     1141 gcaac
//
