LOCUS       sequence-287088-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3515
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(758..782)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             790..831
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cgcgatccaa ccggcagaag
       61 gagtacaaat gcggggacct ggtgttcgcc aagatgaagg gctacccaca ctggccggcc
      121 cggattgacg agatgcctga ggctgccgtg aaatcaacag ccaacaaata ccaagtcttt
      181 tttttcggga cccacgagac ggcattcctg ggccccaaag acctcttccc ttacgaggaa
      241 tccaaggaga agtttggcaa gcccaacaag aggaaagggt tcagcgaggg gctgtgggag
      301 atcgaaaaca accctactgt caaggcttcc ggctatcagt cctcccagaa aaagagctgt
      361 gtggaagagc ctgaaccaga gcccgaagct gcagagggtg acggtgataa gaaggggaat
      421 gcagagggca gcagcgacga ggaagggaag ctggtcattg atgagccagc caaggagaag
      481 aacgagaaag gagcgttgaa gaggagagca ggggacttgc tggaggactc tcctaaacgt
      541 cccaaggagg cagaaaaccc tgaaggagag gagaaggagg cagccacctt ggaggttgag
      601 aggccccttc ctatggaggt ggaaaagaat agcaccccct ctgagcccgg ctctggccgg
      661 gggcctcccc aagaggaaga agaagaggag gatgaagagg aagaggctac caaggaagat
      721 gctgaggccc caggcatcag agatcatgag agcctgtacc caactttctt gtacaaagtg
      781 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      841 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      901 gaattgacta ataatctatt tacctc
//
