LOCUS       sequence-287091-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3516
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(773..797)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             805..846
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccgaggacct ctctgcggcc
       61 acgtcctaca ccgaagatga tttctactgc cccgtctgtc aggaggtgct caaaacgccc
      121 gtgcggacca cggcctgtca gcacgttttc tgtagaaaat gtttcctgac tgcaatgagg
      181 gaaagcggag cacattgtcc cctatgtcgt ggaaatgtga ctagaagaga gagagcatgt
      241 cctgaacggg ccttagacct tgaaaatata atgaggaagt tttctggtag ctgcagatgc
      301 tgtgcaaaac agattaaatt ctatcgcatg agacatcatt acaaatcttg taagaagtat
      361 caggatgaat atggtgtttc ttctatcatt ccaaactttc agatctctca agattcagta
      421 gggaacagca ataggagtga aacatccaca tctgataaca cagaaactta ccaagagaat
      481 acaagttctt ctggtcatcc tacttttaag tgtcccctgt gtcaagaatc aaattttacc
      541 agacagcgtt tactggatca ctgtaacagt aatcacctat ttcagatagt tcctgtgaca
      601 tgtcctattt gtgtgtctct tccttgggga gatcctagcc agattaccag aaatttcgtt
      661 agtcatctaa atcagagaca tcaatttgat tatggagaat ttgtgaatct tcagctagat
      721 gaagaaaccc aataccaaac tgctgttgaa gaatcttttc aagtaaacat ctgcccaact
      781 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
      841 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
      901 tcttgtggaa aggacgaaac ggcggtgcta ggcggggccc a
//
