LOCUS       sequence-287094-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3517
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(986..1010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1018..1059
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggtgcagca gcggggcgcg
       61 agggccaagc gggacggcgg gccgccgccc ccgggacccg ggccggccga ggagggggcg
      121 cgcgagcccg gctggtgcaa gaccccgagc ggccacatca agaggccgat gaacgcattc
      181 atggtgtggt cgcagcacga acggcggaag atcatggacc agtggcccga catgcacaac
      241 gccgagatct ccaagcgcct gggccgccgc tggcagctgc tgcaggactc ggagaagatc
      301 ccgttcgtgc gggaggcgga gcggctgcgg ctcaagcaca tggcggatta cccggactac
      361 aagtaccggc cgcgcaaaaa gagcaagggg gcgcccgcca aggcgcggcc ccgccccccc
      421 ggtggtagcg gtggcggcag ccggctcaag cccgggccgc agctgcctgg ccgcgggggc
      481 cgccgagcag cgggagggcc tttggggggc ggggcggcgg cgcccgagga cgacgatgaa
      541 gacgacgacg aggagctgct ggaagtgcgc ctggtcgaga ccccggggcg ggagctgtgg
      601 aggatggtcc cggcgggacg ggccgctcgg ggacaagcgg agcgcgccca agggccgtcg
      661 ggcgaggggg cggccgccgc cgccgccgcc tccccgacac cgtcggagga cgaggagccg
      721 gaggaagagg aggaggaggc ggcagcggct gaggaaggtg aagaggagac ggtggcgtcg
      781 ggggaggagt cgctgggctt tctgtccagg ctgccccctg gcccggccgg cctggactgc
      841 agcgccctgg atcgcgaccc ggacctgcag cctccctcgg gcacgtcgca cttcgagttc
      901 ccggactact gcacccccga ggttaccgag atgatcgcgg gggactggcg cccgtctagc
      961 atcgcagacc tggttttcac ctacttgcca actttcttgt acaaagtggt tgatatcggt
     1021 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1081 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ataagtggcg
     1141 gtgattaatt acaa
//
