LOCUS       sequence-287097-         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3518
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(446..470)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             478..519
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgatgctgag tccggaccaa
       61 gcagcagatt cggaccatcc cagctcggcg cactcggatc cggagtccct gggcggcacg
      121 gacaccaagg tgctcggcag cgtgtcggac ctggagccgg tggaggaggc cgagggcgac
      181 ggcaagggcg gcagccgagc cgcgctctac ccgcacccgc agcagctgag ccgcgaggag
      241 aagcgccgcc gccggcgcgc cacggccaag taccgctcgg cccacgccac ccgcgagcgc
      301 atccgcgtgg aagccttcaa cttggccttc gccgagctcc gcaaattgct gcccacgctg
      361 cccccggaca agaagctctc caagatcgag atcctgcgcc tggccatctg ctacatctcc
      421 tatctcaacc acgtcctgga cgtgttgcca actttcttgt acaaagtggt tgatatcggt
      481 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
      541 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tccatcaacg
      601 tttcggacat tggt
//
