LOCUS       sequence-28710-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV H2B-RFP Hygro (w512-1)
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(102..788)
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     regulatory      785..794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttgtatc cagaggttga ttgtcgagcg gccgccactg tgctggatat caaccacttt
       61 gtacaagaaa gctgggtcta gatatctcga gtgcggccgc ttcagttggc cttctcgggc
      121 aggtcgctgt accgggccac gctggcctcg tacagctcga agtactcgtc cttcttcttc
      181 cgcagcatct ggagccggat gtcggtgaag tggaagccgg gcatggtcag ggcgcgcacg
      241 gccttcttgc tccggtagct ggtgtagtgg tggcagatca ggtgccggtc gcccaccttc
      301 agggccatca cgttccggcc gcacagcacg ccgttctcgg ggtacaccac ctcggtgctg
      361 ggctcccagc cgccgctctt gttcttcatc acggggccgt cggcggggaa gttggtgccg
      421 tgcaccttca ccttgtagat caggcagttg ccctccaggc tggtgtcctg gtgggcggtc
      481 aggatgccgc cgtcctcgta ggtggtggtt ctctcccagg tgaagccctc ggggaagctc
      541 tgcttgaaga agtcggggat ctcggcggtg tggtgcacga aggtcctgct gccgtactcg
      601 cagcaggggg ccaggatgtc gaaggcgaag ggcagggggg cgccctcggt cacgtggatt
      661 ctcatgctct gggtgccggc gaaggggttg ccgtcgccct cgccctcgca cttgaagtag
      721 tggccgttca cggtgccctc catgtacatc ttgatgcgca tactctcctt cagcaggccg
      781 ctcaccatgg tggcgaccgg gccagctccg gctccagctc cagcgccagc tccggctccg
      841 ttgttggcct tctcgggc
//
