LOCUS       sequence-287103-        2222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3520
FEATURES             Location/Qualifiers
     source          1..2222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2054..2078)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2086..2127
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcatcacca acagcgaatg
       61 gctgccttag ggacggacaa agagctgagt gatttactgg atttcagtgc gatgttttca
      121 cctcctgtga gcagtgggaa aaatggacca acttctttgg caagtggaca ttttactggc
      181 tcaaatgtag aagacagaag tagctcaggg tcctggggga atggaggaca tccaagcccg
      241 tccaggaact atggagatgg gactccctat gaccacatga ccagcaggga ccttgggtca
      301 catgacaatc tctctccacc ttttgtcaat tccagaatac aaagtaaaac agaaaggggc
      361 tcatactcat cttatgggag agaatcaaac ttacagggtt gccaccagca gagtctcctt
      421 ggaggtgaca tggatatggg caacccagga accctttcgc ccaccaaacc tggttcccag
      481 tactatcagt attctagcaa taatccccga aggaggcctc ttcacagtag tgccatggag
      541 gtacagacaa agaaagttcg aaaagttcct ccaggtttgc catcttcagt ctatgctcca
      601 tcagcaagca ctgccgacta caatagggac tcgccaggct atccttcctc caaaccagca
      661 accagcactt tccctagctc cttcttcatg caagatggcc atcacagcag tgacccttgg
      721 agctcctcca gtgggatgaa tcagcctggc tatgcaggaa tgttgggcaa ctcttctcat
      781 attccacagt ccagcagcta ctgtagcctg catccacatg aacgtttgag ctatccatca
      841 cactcctcag cagacatcaa ttccagtctt cctccgatgt ccactttcca tcgtagtggt
      901 acaaaccatt acagcacctc ttcctgtacg cctcctgcca acgggacaga cagtataatg
      961 gcaaatagag gaagcggggc agccggcagc tcccagactg gagatgctct ggggaaagca
     1021 cttgcttcga tctattctcc agatcacact aacaacagct tttcatcaaa cccttcaact
     1081 cctgttggct ctcctccatc tctctcagca ggcacagctg tttggtctag aaatggagga
     1141 caggcctcat cgtctcctaa ttatgaagga cccttacact ctttgcaaag ccgaattgaa
     1201 gatcgtttag aaagactgga tgatgctatt catgttctcc ggaaccatgc agtgggccca
     1261 tccacagcta tgcctggtgg tcatggggac atgcatggaa tcattggacc ttctcataat
     1321 ggagccatgg gtggtctggg ctcagggtat ggaaccggcc ttctttcagc caacagacat
     1381 tcactcatgg tggggaccca tcgtgaagat ggcgtggccc tgagaggcag ccattctctt
     1441 ctgccaaacc aggttccggt tccacagctt cctgtccagt ctgcgacttc ccctgacctg
     1501 aacccacccc aggaccctta cagaggcatg ccaccaggac tacaggggca gagtgtctcc
     1561 tctggcagct ctgagatcaa atccgatgac gagggtgatg agaacctgca agacacgaaa
     1621 tcttcggagg acaagaaatt agatgacgac aagaaggata tcaaatcaat tactaggtca
     1681 agatctagca ataatgacga tgaggacctg acaccagagc agaaggcaga gcgtgagaag
     1741 gagcggagga tggccaacaa tgcccgagag cgtctgcggg tccgtgacat caacgaggct
     1801 ttcaaagagc tcggccgcat ggtgcagctc cacctcaaga gtgacaagcc ccagaccaag
     1861 ctcctgatcc tccaccaggc ggtggccgtc atcctcagtc tggagcagca agtccgagaa
     1921 aggaatctga atccgaaagc tgcgtgtctg aaaagaaggg aggaagagaa ggtgtcctcg
     1981 gagcctcccc ctctctcctt ggccggccca caccctggaa tgggagacgc atcgaatcac
     2041 atgggacaga tgttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     2101 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     2161 tcgatttctt ggctttatat atcttgtgga aaggacgaga ctcggagtct tctaggcaac
     2221 ct
//
