LOCUS       sequence-287106-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3521
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(860..884)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             892..933
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc ccaagtactg cagggcgccg
       61 aactgctcca acactgcggg ccgcctgggt gcagacaacc gccctgtgag cttctacaag
      121 ttcccactga aggatggtcc ccggctgcag gcctggctgc agcacatggg ctgtgagcac
      181 tgggtgccca gctgccacca gcacttgtgc agcgagcact tcacaccctc ctgcttccag
      241 tggcgctggg gtgtgcgcta cctgcggcct gatgcagtgc cctccatctt ctcccgggga
      301 ccacctgcca agagtcagcg gaggacccga agcacccaga agccagtctc gccgccgcct
      361 cccctacaga agaatacacc cctgccccag agccctgcca tcccagtctc tggcccagtg
      421 cgcctagtgg tgctgggccc cacatcgggg agccccaaga ctgtggccac catgctcctg
      481 acccccctgg cccctgcgcc aactcctgag cggtcacaac ctgaagtccc tgcccaacag
      541 gcccagaccg ggctgggccc agtgctggga gcactgcaac gccgggtgcg gaggctgcaa
      601 cggtgccagg agcggcacca ggcgcagctg caggccctgg aacggctggc acagcagcta
      661 cacggggaga gcctgctggc acgggcacgc cggggtctgc agcgcctgac aacagcccag
      721 acccttggac ctgaggaatc ccaaaccttc accatcatct gtggagggcc tgacatagcc
      781 atggtccttg cccaggaccc tgcacctgcc acagtggatg ccaagccgga gctcctggac
      841 actcggatcc ccagtgcata cccaactttc ttgtacaaag tggttgatat cggtaagcct
      901 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      961 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaagatat cttatccttt
     1021 tctttaag
//
