LOCUS       sequence-287109-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3522
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(944..968)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             976..1017
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccagggat cagcagtcag
       61 atcctcacca atgctcaggg acaggttatt ggaacccttc catgggtagt gaactcagct
      121 agtgtggcgg ccccagcacc agcccaaagc ctgcaggtcc aggccgtgac cccccagctg
      181 ttgttgaacg cccagggcca ggtgattgcg accctggcta gcagccccct gcctccacct
      241 gtggctgtcc ggaagccaag cacacctgag tcccctgcta agagtgaggt gcagcccatc
      301 cagcccacac caaccgtgcc ccagcctgct gtggtcattg ccagcccagc tccagccgcc
      361 aagccatctg cctctgctcc tatcccaatt acctgctcag agacccccac cgtcagccag
      421 ttggtgtcca agccacatac tccaagtctg gatgaggatg ggatcaactt agaagagatc
      481 cgggagtttg ccaagaactt taagatccgg cggctctcgc tgggccttac acagacccag
      541 gtgggtcagg ctctgactgc aacggaaggt ccagcctaca gccagtcagc catctgccgg
      601 ttcgagaagc tagacatcac acccaagagt gcccagaagc taaagccggt gctggaaaag
      661 tggctaaacg aagctgaact gcggaaccag gaaggccagc agaacctgat ggagtttgtg
      721 ggaggcgagc cctccaagaa acgcaaacgc cgcacctcct tcacccccca ggccatagag
      781 gctctcaatg cctattttga gaagaaccca ctgcccacag gccaggagat cactgaaatt
      841 gctaaggagc tcaactacga ccgtgaggta gtgcgggtct ggttctgcaa tcggcgccag
      901 acgctcaaga acaccagcaa gctgaacgtc tttcagatcc ctttgccaac tttcttgtac
      961 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1021 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1081 aaggacgagt cactgttagt tgagaaccac ag
//
