LOCUS       sequence-28711-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV H2B-RFP Hygro (w512-1)
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..106
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             142..519
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggcgtganc atcacgctnn ttttgacctc catagaagac accgactcta gntccagtgt
       61 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa aggaaccaat
      121 tcagtcgacg gtaccgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag
      181 ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc
      241 cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc
      301 ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc
      361 atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg
      421 gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc
      481 gagggtacta aggccatcac caagtacacc agcgctaagg atccaccggt cgccaccatg
      541 gtgagcggcc tgctgaagga gagtatgcgc atcaagatgt acatggaggg caccgtgaac
      601 ggccactact tcaagtgcga gggcgagggc gacggcaacc ccttcgccgg cacccagagc
      661 atgagaatcc acgtgaccga gggcgccccc ctgcccttcg ccttcgacat cctggccccc
      721 tgctgcgagt acggcagcag gaccttcgtg caccacacng ccgagatccc gacttcttca
      781 gc
//
