LOCUS       sequence-287112-        2819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3523
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2651..2675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2683..2724
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgaggaatt tgtgaccctc
       61 aaggatgtcg gcatggactt caccttggga gactgggagc agctcgggct ggaacagggg
      121 gacacgttct gggacacagc gttggacaat tgccaggacc tcttcctgct ggacccccca
      181 agacccaacc tgacctccca cccagatggc agtgaagatc tggagcctct ggcaggagga
      241 agcccagaag caacaagccc tgatgtgact gagaccaaga actctcctct gatggaggat
      301 ttcttcgaag aaggattctc ccaggagatt atagagatgt tatccaagga tggcttctgg
      361 aactccaatt tcggagaagc ctgtatagag gacacctggt tagatagttt gctaggcgat
      421 ccagaaagtc ttctgaggtc tgatattgcc accaacgggg aaagtcccac ggaatgcaag
      481 agtcatgaat taaagagagg actcagtcct gtgtccaccg tttccacggg agaagattcc
      541 atggtgcata atgtttctga aaagaccctc acaccagcta agtctaagga atataggggt
      601 gagtttttct cctactccga ccacagccag caggattctg ttcaggaagg ggagaaacca
      661 tatcaatgta gtgaatgtgg gaaaagcttc agtgggagtt accgtcttac ccagcactgg
      721 atcactcata ctagggagaa acccactgtc catcaagagt gtgagcaagg ttttgaccgg
      781 aatgcttccc tttctgtgta tccgaaaact cacacgggct acaaattcta tgtgtgtaat
      841 gaatatggga caacttttag tcagagtaca tacctgtggc atcagaaaac tcacactgga
      901 gaaaaaccat gtaagagtca agatagtgac cacccaccca gtcatgacac acagcctggt
      961 gagcatcaga aaactcacac agatagtaag tcctacaact gtaacgaatg cggcaaggct
     1021 tttacccgga tcttccacct tactcggcac cagaagatcc acactcggaa acgctatgag
     1081 tgttccaagt gccaggcgac cttcaacttg agaaaacacc tcatccaaca tcagaaaact
     1141 cacgctgcaa aaactacctc tgagtgtcag gagtgtggga agatttttag gcacagttcg
     1201 ctgctcattg aacaccaggc tcttcatgct ggagaggagc cttataagtg taacgaacgt
     1261 gggaaatcct tcaggcataa ctctacccta aagatccatc agagggttca cagtggagag
     1321 aagccttaca aatgcagtga gtgtgggaag gccttccacc ggcacactca ccttaatgaa
     1381 catcggcgaa ttcatacagg ctacagaccc cacaaatgtc aggaatgcgt caggagtttc
     1441 agccggccct cacatctgat gcgacatcag gccattcaca ccgcagaaaa gccctatagc
     1501 tgtgctgaat gcaaggagac tttcagcgat aacaatcgcc ttgtgcaaca ccagaaaatg
     1561 cacactgtca aaaccccata tgaatgtcag gagtgcggag aacgcttcat ttgcggctca
     1621 accctgaagt gccacgagag tgttcacgcc agagaaaaac aaggattttt tgtgagtggg
     1681 aagatcttgg atcagaaccc agaacagaaa gagaagtgct ttaagtgtaa caaatgtgag
     1741 aaaaccttta gctgcagcaa atacctaact cagcacgaga ggattcacac caggggagtg
     1801 aagccctttg aatgtgacca gtgtgggaaa gcctttggcc aaagtactcg gctcattcac
     1861 catcaaagaa tccactctag agtgaggctg tataaatggg gtgagcaagg gaaagccatc
     1921 agcagtgcct cccttatcaa acttcagtcc ttccacacaa aggagcaccc ttttaaatgt
     1981 aacgaatgcg gaaagacctt cagccacagt gcacacctct caaaacatca gttaattcac
     2041 gctggagaga atccctttaa atgtagtaag tgtgacagag tcttcaccca gagaaactac
     2101 cttgttcagc atgagcgaac tcatgccaga aagaagccgt tggtgtgtaa cgaatgcggg
     2161 aaaacgttcc gtcagagctc atgcctttct aagcatcaga gaattcactc aggtgagaag
     2221 ccctatgtat gtgattactg cgggaaggcc ttcggcctga gtgctgagct tgtccgccac
     2281 cagagaattc acactggaga aaagccttat gtttgtcagg aatgcgggaa agccttcacc
     2341 cagagctcat gcctttctat tcaccggaga gttcacactg gggagaagcc ctacagatgt
     2401 ggtgaatgtg ggaaagcctt tgcccagaaa gcaaatctaa cacagcacca gagaattcac
     2461 acaggggaga agccttactc ctgtaatgtg tgtggcaaag cttttgtcct cagtgcccat
     2521 ctcaaccagc acctgagagt tcacacccag gagacacttt atcagtgtca acgttgccag
     2581 aaagcctttc ggtgccactc gagcctcagc cgccatcagc gtgtacacaa caagcagcaa
     2641 tactgcctgt acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     2701 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2761 atttcttggc tttatatatc ttgtggaaag gacgaatccg agggcgaaca ccgcacggc
//
