LOCUS       sequence-287118-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3525
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(722..746)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             754..795
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cgaccaattg cgctgcggcg
       61 ggctgtgcca ctacctacaa caagcacatt aacatcagct tccacaggtt tcctttggat
      121 cctaaaagaa gaaaagaatg ggttcgcctg gttaggcgca aaaattttgt gccaggaaaa
      181 cacacttttc tttgttcaaa gcactttgaa gcctcctgtt ttgacctaac aggacaaact
      241 cgacgactta aaatggatgc tgttccaacc atttttgatt tttgtaccca tataaagtct
      301 atgaaactca agtcaaggaa tcttttgaag aaaaacaaca gttgttctcc agctggacca
      361 tctaatttaa aatcaaacat tagtagtcag caagtactac ttgaacacag ctatgccttt
      421 aggaatccta tggaggcaaa aaagaggatc attaaactgg aaaaagaaat agcaagctta
      481 agaagaaaaa tgaaaacttg cctacaaaag gaacgcagag caactcgaag atggatcaaa
      541 gccacgtgtt tggtaaagaa tttagaagca aatagtgtat tacctaaagg tacatcagaa
      601 cacatgttac caactgcctt aagcagtctt cctttggaag attttaagat ccttgaacaa
      661 gatcaacaag ataaaacact gctaagtcta aatctaaaac agaccaagag taccttcatt
      721 tacccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
      781 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
      841 ctttatatat cttgtggaaa ggacgagcct actccgcggc aaccgcggtg
//
