LOCUS       sequence-28712-e         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR/pTER shATM #2 (730-2)
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     270..289
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    335..353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(484..508)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatttnattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat
      121 ccggtaccga attcgaacgc tgacgtcatc aacccgctcc aaggaatcgc gggcccagtg
      181 tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag gaagatggct gtgagggaca
      241 ggggagtggc gccctgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac
      301 gtgaaatgtc tttggatttg ggaatcttat aagttcccta tcagtgatag agatccctga
      361 agatggngct cataaattca agagatttat gagcaccatc ttcatttttg gaaaagcttg
      421 ntaccgagct cggatccact antccagtgt gnaggaattc gcggncgnac tcgagatatc
      481 tagacccagc tttcttgtac aaagttggca ttacaa
//
