LOCUS       sequence-287120-       10756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3526
FEATURES             Location/Qualifiers
     source          1..10756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4118..4142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6429..6453)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6461..6502
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6613..7201
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6666..6686)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7084..7095)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7273..7506
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7584..7705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7621..7640)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7675..7694
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7745..7880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7900..7919)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7901..7919)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7929..7946)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7929..7945)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7938..7960)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8087..8542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8174..8193)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8384..8405
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8568..8672
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8673..8741,8742..9533)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8891..8910)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9704..10292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10193..10212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10446..10463
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10580..10601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10616..10621,10622..10639,10640..10646)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10654..10670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10659..10681
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10678..10694
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10678..10694
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10713..10733
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10715..10733
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     4141 caccatgccg aacttctgcg ctgcccccaa ctgcacgcgg aagagcacgc agtccgactt
     4201 ggccttcttc aggttcccgc gggaccctgc cagatgccag aagtgggtgg agaactgtag
     4261 gagagcagac ttagaagata aaacacctga tcagctaaat aaacattatc gattatgtgc
     4321 caaacatttt gagacctcta tgatctgtag aactagtcct tataggacag ttcttcgaga
     4381 taatgcaata ccaacaatat ttgatcttac cagtcatttg aacaacccac atagtagaca
     4441 cagaaaacga ataaaagaac tgagtgaaga tgaaatcagg acactgaaac agaaaaaaat
     4501 tgatgaaact tctgagcagg aacaaaaaca taaagaaacc aacaatagca atgctcagaa
     4561 ccccagcgaa gaagagggtg aagggcaaga tgaggacatt ttacctctaa cccttgaaga
     4621 gaaggaaaac aaagaatacc taaaatctct atttgaaatc ttgattctga tgggaaagca
     4681 aaacatacct ctggatggac atgaggctga tgaaatccca gaaggtctct ttactccaga
     4741 taactttcag gcactgctgg agtgtcggat aaattctggt gaagaggttc tgagaaagcg
     4801 gtttgagaca acagcagtta acacgttgtt ttgttcaaaa acacagcaga ggcagatgct
     4861 agagatctgt gagagctgta ttcgagaaga aactctcagg gaagtgagag actcacactt
     4921 cttttccatt atcactgacg atgtagtgga catagcaggg gaagagcacc tacctgtgtt
     4981 ggtgaggttt gttgatgaat ctcataacct aagagaggaa tttataggct tcctgcctta
     5041 tgaagccgat gcagaaattt tggctgtgaa atttcacact atgataactg agaagtgggg
     5101 attaaatatg gagtattgtc gtggccaggc ttacattgtc tctagtggat tttcttccaa
     5161 aatgaaagtt gttgcttcta gacttttaga gaaatatccc caagctatct acacactctg
     5221 ctcttcctgt gccttaaata tgtggttggc aaaatcagta cctgttatgg gagtatctgt
     5281 tgcattagga acaattgagg aagtttgttc ttttttccat cgatcaccac aactgctttt
     5341 agaacttgac aacgtaattt ctgttctttt tcagaacagt aaagaaaggg gtaaagaact
     5401 gaaggaaatc tgccattctc agtggacagg caggcatgat gcttttgaaa ttttagtgga
     5461 actcctgcaa gcacttgttt tatgtttaga tggtataaat agtgacacaa atattagatg
     5521 gaataactat atagctggcc gagcatttgt actctgcagt gcagtgtcag attttgattt
     5581 cattgttact attgttgttc ttaaaaatgt cctatctttt acaagagcct ttgggaaaaa
     5641 cctccagggg caaacctctg atgtcttctt tgcggccggt agcttgactg cagtactgca
     5701 ttcactcaac gaagtgatgg aaaatattga agtttatcat gaattttggt ttgaggaagc
     5761 cacaaatttg gcaaccaaac ttgatattca aatgaaactc cctgggaaat tccgcagagc
     5821 tcaccagggt aacttggaat ctcagctaac ctctgagagt tactataaag aaaccctaag
     5881 tgtcccaaca gtggagcaca ttattcagga acttaaagat atattctcag aacagcacct
     5941 caaagctctt aaatgcttat ctctggtacc ctcagtcatg ggacaactca aattcaatac
     6001 gtcggaggaa caccatgctg acatgtatag aagtgactta cccaatcctg acacgctgtc
     6061 agctgagctt cattgttgga gaatcaaatg gaaacacagg gggaaagata tagagcttcc
     6121 gtccaccatc tatgaagccc tccacctgcc tgacatcaag ttttttccta atgtgtatgc
     6181 attgctgaag gtcctgtgta ttcttcctgt gatgaaggtt gagaatgagc ggtatgaaaa
     6241 tggacgaaag cgtcttaaag catatttgag gaacactttg acagaccaaa ggtcaagtaa
     6301 cttggctttg cttaacataa attttgatat aaaacacgac ctggatttaa tggtggacac
     6361 atatattaaa ctctatacaa gtaagtcaga gcttcctaca gataattccg aaactgtgga
     6421 aaataccttg ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc
     6481 tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat
     6541 ttcttggctt tatatatctt gtggaaagga cgaggcggtg ttcccagctc ggccgtgacg
     6601 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6661 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6721 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6781 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6841 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6901 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6961 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7021 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7081 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7141 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7201 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7261 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7321 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7381 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7441 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7501 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7561 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7621 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7681 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     7741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7801 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7861 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     7921 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     7981 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8041 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     8101 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8161 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8221 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8281 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8341 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8401 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8461 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8521 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8581 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8641 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8701 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8761 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8821 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8881 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8941 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9001 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9061 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9121 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9181 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9241 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9301 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9361 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9421 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9481 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9541 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9601 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9841 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9901 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9961 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10021 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10081 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10141 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10201 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10261 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10321 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10381 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10441 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10501 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    10561 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    10621 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    10681 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    10741 agctggagct gcaagc
//
