LOCUS       sequence-287121-        2492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3526
FEATURES             Location/Qualifiers
     source          1..2492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2324..2348)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2356..2397
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccgaactt ctgcgctgcc
       61 cccaactgca cgcggaagag cacgcagtcc gacttggcct tcttcaggtt cccgcgggac
      121 cctgccagat gccagaagtg ggtggagaac tgtaggagag cagacttaga agataaaaca
      181 cctgatcagc taaataaaca ttatcgatta tgtgccaaac attttgagac ctctatgatc
      241 tgtagaacta gtccttatag gacagttctt cgagataatg caataccaac aatatttgat
      301 cttaccagtc atttgaacaa cccacatagt agacacagaa aacgaataaa agaactgagt
      361 gaagatgaaa tcaggacact gaaacagaaa aaaattgatg aaacttctga gcaggaacaa
      421 aaacataaag aaaccaacaa tagcaatgct cagaacccca gcgaagaaga gggtgaaggg
      481 caagatgagg acattttacc tctaaccctt gaagagaagg aaaacaaaga atacctaaaa
      541 tctctatttg aaatcttgat tctgatggga aagcaaaaca tacctctgga tggacatgag
      601 gctgatgaaa tcccagaagg tctctttact ccagataact ttcaggcact gctggagtgt
      661 cggataaatt ctggtgaaga ggttctgaga aagcggtttg agacaacagc agttaacacg
      721 ttgttttgtt caaaaacaca gcagaggcag atgctagaga tctgtgagag ctgtattcga
      781 gaagaaactc tcagggaagt gagagactca cacttctttt ccattatcac tgacgatgta
      841 gtggacatag caggggaaga gcacctacct gtgttggtga ggtttgttga tgaatctcat
      901 aacctaagag aggaatttat aggcttcctg ccttatgaag ccgatgcaga aattttggct
      961 gtgaaatttc acactatgat aactgagaag tggggattaa atatggagta ttgtcgtggc
     1021 caggcttaca ttgtctctag tggattttct tccaaaatga aagttgttgc ttctagactt
     1081 ttagagaaat atccccaagc tatctacaca ctctgctctt cctgtgcctt aaatatgtgg
     1141 ttggcaaaat cagtacctgt tatgggagta tctgttgcat taggaacaat tgaggaagtt
     1201 tgttcttttt tccatcgatc accacaactg cttttagaac ttgacaacgt aatttctgtt
     1261 ctttttcaga acagtaaaga aaggggtaaa gaactgaagg aaatctgcca ttctcagtgg
     1321 acaggcaggc atgatgcttt tgaaatttta gtggaactcc tgcaagcact tgttttatgt
     1381 ttagatggta taaatagtga cacaaatatt agatggaata actatatagc tggccgagca
     1441 tttgtactct gcagtgcagt gtcagatttt gatttcattg ttactattgt tgttcttaaa
     1501 aatgtcctat cttttacaag agcctttggg aaaaacctcc aggggcaaac ctctgatgtc
     1561 ttctttgcgg ccggtagctt gactgcagta ctgcattcac tcaacgaagt gatggaaaat
     1621 attgaagttt atcatgaatt ttggtttgag gaagccacaa atttggcaac caaacttgat
     1681 attcaaatga aactccctgg gaaattccgc agagctcacc agggtaactt ggaatctcag
     1741 ctaacctctg agagttacta taaagaaacc ctaagtgtcc caacagtgga gcacattatt
     1801 caggaactta aagatatatt ctcagaacag cacctcaaag ctcttaaatg cttatctctg
     1861 gtaccctcag tcatgggaca actcaaattc aatacgtcgg aggaacacca tgctgacatg
     1921 tatagaagtg acttacccaa tcctgacacg ctgtcagctg agcttcattg ttggagaatc
     1981 aaatggaaac acagggggaa agatatagag cttccgtcca ccatctatga agccctccac
     2041 ctgcctgaca tcaagttttt tcctaatgtg tatgcattgc tgaaggtcct gtgtattctt
     2101 cctgtgatga aggttgagaa tgagcggtat gaaaatggac gaaagcgtct taaagcatat
     2161 ttgaggaaca ctttgacaga ccaaaggtca agtaacttgg ctttgcttaa cataaatttt
     2221 gatataaaac acgacctgga tttaatggtg gacacatata ttaaactcta tacaagtaag
     2281 tcagagcttc ctacagataa ttccgaaact gtggaaaata ccttgccaac tttcttgtac
     2341 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     2401 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2461 aaggacgagg cggtgttccc agctcggccg tg
//
