LOCUS       sequence-287127-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3528
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(497..521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             529..570
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggccaacct ggagcgcacc
       61 ttcatcgcca tcaagccgga cggcgtgcag cgcggcctgg tgggcgagat catcaagcgc
      121 ttcgagcaga agggattccg cctcgtggcc atgaagttcc tccgggcctc tgaagaacac
      181 ctgaagcagc actacattga cctgaaagac cgaccattct tccctgggct ggtgaagtac
      241 atgaactcag ggccggttgt ggccatggtc tgggaggggc tgaacgtggt gaagacaggc
      301 cgagtgatgc ttggggagac caatccagca gattcaaagc caggcaccat tcgtggggac
      361 ttctgcattc aggttggcag gaacatcatt catggcagtg attcagtaaa aagtgctgaa
      421 aaagaaatca gcctatggtt taagcctgaa gaactggttg actacaagtc ttgtgctcat
      481 gactgggtct atgaattgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      541 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      601 atttcgattt cttggcttta tatatcttgt ggaaaggacg accaaggcat cgcgtctcaa
      661 agtaa
//
