LOCUS       sequence-28713-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X1 Zeo/pTER shATM #2 (735-1)
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(168..192)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(276..296)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(694..705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gattgggatc ttataagttc cctatcagtg atagagatcc ctgaagatgg tgctcataaa
       61 ttcaagagat ttatgagcac catcttcatt tttggaaaag cttggtaccg agctcggatc
      121 cactagtcca gtgtggtgga attcgcggcc gcactcgaga tatctagacc cagctttctt
      181 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac
      241 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
      301 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
      361 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
      421 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
      481 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
      541 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
      601 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
      661 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
      721 tcc
//
