LOCUS       sequence-287130-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3529
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1319..1343)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1351..1392
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aggcaatggc ggccagcact
       61 tccctgcctg accctggaga ctttgaccgg aacgtgcccc ggatctgtgg ggtgtgtgga
      121 gaccgagcca ctggctttca cttcaatgct atgacctgtg aaggctgcaa aggcttcttc
      181 aggcgaagca tgaagcggaa ggcactattc acctgcccct tcaacgggga ctgccgcatc
      241 accaaggaca accgacgcca ctgccaggcc tgccggctca aacgctgtgt ggacatcggc
      301 atgatgaagg agttcattct gacagatgag gaagtgcaga ggaagcggga gatgatcctg
      361 aagcggaagg aggaggaggc cttgaaggac agtctgcggc ccaagctgtc tgaggagcag
      421 cagcgcatca ttgccatact gctggacgcc caccataaga cctacgaccc cacctactcc
      481 gacttctgcc agttccggcc tccagttcgt gtgaatgatg gtggagggag ccatccttcc
      541 aggcccaact ccagacacac tcccagcttc tctggggact cctcctcctc ctgctcagat
      601 cactgtatca cctcttcaga catgatggac tcgtccagct tctccaatct ggatctgagt
      661 gaagaagatt cagatgaccc ttctgtgacc ctagagctgt cccagctctc catgctgccc
      721 cacctggctg acctggtcag ttacagcatc caaaaggtca ttggctttgc taagatgata
      781 ccaggattca gagacctcac ctctgaggac cagatcgtac tgctgaagtc aagtgccatt
      841 gaggtcatca tgttgcgctc caatgagtcc ttcaccatgg acgacatgtc ctggacctgt
      901 ggcaaccaag actacaagta ccgcgtcagt gacgtgacca aagccggaca cagcctggag
      961 ctgattgagc ccctcatcaa gttccaggtg ggactgaaga agctgaactt gcatgaggag
     1021 gagcatgtcc tgctcatggc catctgcatc gtctccccag atcgtcctgg ggtgcaggac
     1081 gccgcgctga ttgaggccat ccaggaccgc ctgtccaaca cactgcagac gtacatccgc
     1141 tgccgccacc cgcccccggg cagccacctg ctctatgcca agatgatcca gaagctagcc
     1201 gacctgcgca gcctcaatga ggagcactcc aagcagtacc gctgcctctc cttccagcct
     1261 gagtgcagca tgaagctaac gccccttgtg ctcgaagtgt ttggcaatga gatctcctgc
     1321 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1381 ctcgattcta cgtagtaatg aactagcccg taacttgaaa gtatttcgat ttcttggctt
     1441 tatatatctt gtggaaagga cgagtttctt caagctgcgc ttccaat
//
