LOCUS       sequence-287133-        2333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3530
FEATURES             Location/Qualifiers
     source          1..2333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2165..2189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2197..2238
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagagacact ggagttcaac
       61 gacgtctatc aggaggtgaa aggttccatg aatgatggtc gactgaggtt gagccgtcag
      121 ggcatcatct tcaagaatag caagacaggc aaagtggaca acatccaggc tggggagtta
      181 acagaaggta tctggcgccg tgttgctctg ggccatggac ttaaactgct tacaaagaat
      241 ggccatgtct acaagtatga tggcttccga gaatcggagt ttgagaaact ctctgatttc
      301 ttcaaaactc actatcgcct tgagctaatg gagaaggacc tttgtgtgaa gggctggaac
      361 tgggggacag tgaaatttgg tgggcagctg ctttcctttg acattggtga ccagccagtc
      421 tttgagatac ccctcagcaa tgtgtcccag tgcaccacag gcaagaatga ggtgacactg
      481 gaattccacc aaaacgatga cgcagaggtg tctctcatgg aggtgcgctt ctacgtccca
      541 cccacccagg aggatggtgt ggaccctgtt gaggcctttg cccagaatgt gttgtcaaag
      601 gcggatgtaa tccaggccac gggagatgcc atctgcatct tccgggagct gcagtgtctg
      661 actcctcgtg gtcgttatga cattcggatc taccccacct ttctgcacct gcatggcaag
      721 acctttgact acaagatccc ctacaccaca gtactgcgtc tgtttttgtt accccacaag
      781 gaccagcgcc agatgttctt tgtgatcagc ctggatcccc caatcaagca aggccaaact
      841 cgctaccact tcctgatcct cctcttctcc aaggacgagg acatttcgtt gactctgaac
      901 atgaacgagg aagaagtgga gaagcgcttt gagggtcggc tcaccaagaa catgtcagga
      961 tccctctatg agatggtcag ccgggtcatg aaagcactgg taaaccgcaa gatcacagtg
     1021 ccaggcaact tccaagggca ctcaggggcc cagtgcatta cctgttccta caaggcaagc
     1081 tcaggactgc tctacccgct ggagcggggc ttcatctacg tccacaagcc acctgtgcac
     1141 atccgcttcg atgagatctc ctttgtcaac tttgctcgtg gtaccactac tactcgttcc
     1201 tttgactttg aaattgagac caagcagggc actcagtata ccttcagcag cattgagagg
     1261 gaggagtacg ggaaactgtt tgattttgtc aacgcgaaaa agctcaacat caaaaaccga
     1321 ggattgaaag agggcatgaa cccaagctac gatgaatatg ctgactctga tgaggaccag
     1381 catgatgcct acttggagag gatgaaggag gaaggcaaga tccgggagga gaatgccaat
     1441 gacagcagcg atgactcagg agaagaaacc gatgagtcat tcaacccagg tgaagaggag
     1501 gaagatgtgg cagaggagtt tgacagcaac gcctctgcca gctcctccag taatgagggt
     1561 gacagtgacc gggatgagaa gaagcggaaa cagctcaaaa aggccaagat ggccaaggac
     1621 cgcaagagcc gcaagaagcc tgtggaggtg aagaagggca aagaccccaa tgcccccaag
     1681 aggcccatgt ctgcatacat gctgtggctc aatgccagcc gagagaagat caagtcagac
     1741 catcctggca tcagcatcac ggatctttcc aagaaggcag gcgagatctg gaagggaatg
     1801 tccaaagaga agaaagagga gtgggatcgc aaggctgagg atgccaggag ggactatgaa
     1861 aaagccatga aagaatatga agggggccga ggcgagtctt ctaagaggga caagtcaaag
     1921 aagaagaaga aagtaaaggt aaagatggaa aagaaatcca cgccctctag gggctcatca
     1981 tccaagtcgt cctcaaggca gctaagcgag agcttcaaga gcaaagagtt tgtgtctagt
     2041 gatgagagct cttcgggaga gaacaagagc aaaaagaaga ggaggaggag cgaggactct
     2101 gaagaagaag aactagccag tactcccccc agctcagagg actcagcgtc aggatccgat
     2161 gagtacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     2221 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2281 tggctttata tatcttgtgg aaaggacgaa gctctgtatg cgtacgataa caa
//
