LOCUS       sequence-287139-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3532
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(791..815)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             823..864
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggccgaagg cggggctagc
       61 aaaggtggtg gagaagagcc cgggaagctg ccggagccgg cagaggagga atcccaggtt
      121 ttgcgcggaa ctggccactg taagtggttc aatgtgcgca tgggatttgg attcatctcc
      181 atgataaacc gagagggaag ccccttggat attccagtcg atgtatttgt acaccaaagc
      241 aaactattca tggaaggatt tagaagccta aaagaaggag aaccagtgga attcacattt
      301 aaaaaatctt ccaaaggcct tgagtcaata cgggtaacag gacctggtgg gagcccctgt
      361 ttaggaagtg aaagaagacc caaagggaag acactacaga aaagaaaacc aaagggagat
      421 agatgctaca actgtggtgg ccttgatcat catgctaagg aatgtagtct acctcctcag
      481 ccaaagaagt gccattactg tcagagcatc atgcacatgg tggcaaactg cccacataaa
      541 aatgttgcac agccacccgc gagttctcag ggaagacagg aagcagaatc ccagccatgc
      601 acttcaactc tccctcgaga agtgggaggc gggcatggct gtacatcacc accgtttcct
      661 caggaggcta gggcagagat ctcagaacgg tcaggcaggt cacctcaaga agcttcctcc
      721 acgaagtcat ctatagcacc agaagagcaa agcaaaaagg ggccttcagt tcaaaaaagg
      781 aaaaagacat tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
      841 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
      901 atttcttggc tttatatatc ttgtggaaag gacgagatac gtcattcgaa atcctgcag
//
