LOCUS       sequence-28714-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X2 Zeo/pTER shGFP #2 (790-1)
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(170..194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(330..704)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ggattgggat cttataagtt ccctatcagt gatagagatc cccgctggag tacaactaca
       61 acttcaagag agttgtagtt gtactccagc tttttggaaa agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattcgcgg ccgcactcga gatatctaga cccagctttc
      181 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctagc ccttccagtc
      241 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
      301 aaggtaccat ttaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgcacg
      361 cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg
      421 gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg
      481 tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg
      541 tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc
      601 tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg
      661 tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc
      721 ttgtcgtatt atactatgcc gatatactat gccgatgata attgtcaaca ggggagtttt
      781 tgcaaagcta ggctcaaaaa gctctcatac tctg
//
