LOCUS       sequence-287142-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3533
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(662..686)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             694..735
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ccagcgagcc tccccctcct
       61 tatcctgggg gccccacagc cccacttctg gaagagaaaa gtggagcccc gcccacccca
      121 ggccgttcct ccccagctgt gatgcagccc cctccaggca tgccactgcc ccctgcggac
      181 attggccccc caccctatga gccgccgggt cacccaatgc cccagcctgg cttcatccca
      241 ccacacatga gtgcagatgg cacctacatg cctccgggtt tctaccctcc tccaggcccc
      301 cacccaccca tgggctacta ccccccaggg ccctacacgc cagggcccta ccctggccct
      361 gggggccaca cagccacagt cctggtccct tcaggagctg ccaccacggt gacagtgctg
      421 cagggagaga tctttgaggg agcgcctgtg cagacggtgt gtccccactg ccagcaggcc
      481 atcaccacca agatctccta cgagattggc ttgatgaatt tcgtgctggg tttcttctgt
      541 tgcttcatgg gatgtgatct gggctgctgc ctgatcccct gcctcatcaa tgacttcaag
      601 gatgtgacgc acacatgccc cagctgcaaa gcctacatct acacgtacaa gcgcctgtgc
      661 tagccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
      721 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
      781 ctttatatat cttgtggaaa ggacgagtct tggccgagat cgtttgctgc
//
