LOCUS       sequence-287145-        1304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3534
FEATURES             Location/Qualifiers
     source          1..1304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1136..1160)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1168..1209
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggggaggaa aaaaatccag
       61 atctcccgca tcctggacca aaggaatcgg caggtgacgt tcaccaagcg gaagttcggg
      121 ctgatgaaga aggcctatga gctgagcgtg ctctgtgact gtgagatagc cctcatcatc
      181 ttcaacagcg ccaaccgcct cttccagtat gccagcacgg acatggaccg tgtgctgctg
      241 aagtacacag agtacagcga gccccacgag agccgcacca acactgacat cctcgagacg
      301 ctgaagcgga ggggcattgg cctcgatggg ccagagctgg agccggatga agggcctgag
      361 gagccaggag agaagtttcg gaggctggca ggcgaagggg gtgatccggc cttgccccga
      421 ccccggctgt atcctgcagc tcctgctatg cccagcccag atgtggtata cggggcctta
      481 ccgccaccag gctgtgaccc cagtgggctt ggggaagcac tgcccgccca gagccgccca
      541 tctcccttcc gaccagcagc ccccaaagcc gggcccccag gcctggtgca ccctctcttc
      601 tcaccaagcc acctcaccag caagacacca cccccactgt acctgccgac ggaagggcgg
      661 aggtcagacc tgcctggtgg cctggctggg ccccgagggg gactaaacac ctccagaagc
      721 ctctacagtg gcctgcagaa cccctgctcc actgcaactc ccggaccccc actggggagc
      781 ttccccttcc tccccggagg ccccccagtg ggggccgaag cctgggcgag gagggtcccc
      841 caacccgcgg cgcctccccg ccgacccccc cagtcagcat caagtctgag cgcctctctc
      901 cggcccccgg gggccccggc gactttccta agaccttccc ctatcccttg ctcctcgccc
      961 ggtccctggc agagcctctg cggcctgggc ccgccctgcg ccggctgccc ttggccgacg
     1021 gctggccccg gtaggagatc acccggtggc accagcccag agcgctcgcc aggtacggcg
     1081 agggcacgtg gggaccccac ctccctccag gcctcttcag agaagaccca acagttgcca
     1141 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1201 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1261 atatcttgtg gaaaggacga gatgttccgc cacacgctgc taag
//
