LOCUS       sequence-287148-         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3535
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(416..440)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             448..489
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tctctagcga ccggcccgtg
       61 tcactggagg acgaggtctc ccatagtatg aaggagatga ttggaggctg ttgcgtttgc
      121 tcagacgaga gaggctgggc cgagaacccg ctggtttatt gcgacgggca cggctgcagc
      181 gtcgcggtgc atcaagcttg ctatggcatt gttcaagtac ccactggacc gtggttttgc
      241 aggaaatgtg aatctcagga gagagcagcc agagtggcag agtctcgctc tgttgcccag
      301 gctaaagtgc agtggtgtga tctcagccca ctgcaacctc tgctccccgg gttcaagcga
      361 ttctcctgcc tcagcctccc aaatggtatg caattcctgt tggttagcct catctgccca
      421 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
      481 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
      541 atatcttgtg gaaaggacga cccttaactc aagaatggta tgtg
//
