LOCUS       sequence-28715-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X2 Zeo/pTER shGFP #1 (802-1)
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(169..193)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(329..703)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(722..769)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gattgggaat cttataagtt ccctatcagt gatagagatc ccgaagcagc acgacttctt
       61 cttcaagaga gaagaagtcg tgctgcttct ttttggaaaa gcttggtacc gagctcggat
      121 ccactagtcc agtgtggtgg aattcgcggc cgcactcgag atatctagac ccagctttct
      181 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagcc cttccagtcc
      241 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
      301 aggtaccatt taaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc
      361 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
      421 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
      481 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
      541 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
      601 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
      661 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag ttcctcacct
      721 tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca gggggagctt
      781 tt
//
