LOCUS       sequence-287151-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3536
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(803..827)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             835..876
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tcatgagttc gtatatggtg
       61 aactccaagt atgtggaccc caagttccct ccgtgcgagg agtatttgca gggcggctac
      121 ctaggcgagc agggcgccga ctactacggc ggcggcgcgc agggcgcaga cttccagccc
      181 ccggggctct acccacggcc cgacttcggt gagcagcctt tcggaggcag cggccccggg
      241 cctggctcgg cgctgcctgc gcggggtcac ggacaagagc caggcggccc cggcggtcac
      301 tacgccgctc caggagagcc ttgcccagct cccccggcgc ctccgccggc gcccctgcct
      361 ggcgcccggg cctacagtca gtccgacccc aagcagccgc cctccgggac ggcactcaag
      421 cagccggccg tggtctaccc ctggatgaag aaggtgcacg tgaattcggt gaaccccaac
      481 tacaccggtg gggaacccaa gcggtcccga acggcctaca cccggcagca agtcctagaa
      541 ctggaaaaag aatttcattt taacaggtat ctgacaaggc gccgtcggat tgaaatcgct
      601 cacaccctgt gtctgtcgga gcgccagatc aagatctggt tccagaaccg gaggatgaag
      661 tggaaaaaag atcataagct gcccaacact aaaggcaggt catcgtcctc atcttcctcc
      721 tcatcttgct cctcctcagt cgcccccagc cagcatttac agccgatggc caaagaccac
      781 cacacggacc tgacgacctt atacccaact ttcttgtaca aagtggttga tatcggtaag
      841 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
      901 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacgg tactgctata
      961 taagctattt t
//
