LOCUS       sequence-287154-        1391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3537
FEATURES             Location/Qualifiers
     source          1..1391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1223..1247)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1255..1296
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gcgggggcaa gaagaagagt
       61 agtttccaaa tcaccagcgt caccacggac tatgagggcc ctgggagccc aggggcttcg
      121 gatcccccta ccccacagcc cccaaccggg cccccgcccc gcctgcccaa tggggagccc
      181 agccccgatc cggggggcaa gggcaccccc cggaatggct ccccaccacc tggggcccct
      241 tcctcccgtt tccgggtggt gaagctgccc cacggcctgg gagagcctta tcgccgcggt
      301 cgctggacgt gtgtggatgt ttatgagcga gacctggagc cccacagctt cggcggactc
      361 ctggagggaa ttcgaggggc ctcagggggc gccgggggca gatctttgga ttccaggttg
      421 gagctggcca gcctcggcct gggcgccccc accccaccgt caggcctgtc tcagggcccc
      481 acctcctggc tccgtccacc ccccacctct cctggacctc aggcccgctc cttcactggg
      541 ggactgggcc agctggtggt gcccagcaaa gccaaggcag agaaaccccc actgtcggcc
      601 tcctcacccc agcagcgccc cccagagcct gagaccggtg agagtgcggg cacatcccgg
      661 gctgccacgc ccctgccctc tctgagggtg gaagcggagg ctgggggctc aggggccagg
      721 acccctccac tgtcccggag gaaagctgta gacatgcggc tgcggatgga gttgggtgct
      781 ccagaagaga tggggcaggt gcccccactt gactctcgcc ccagctcccc ggccctctac
      841 ttcacccacg atgccagcct ggttcacaaa tctccagacc ccttcggagc agtagcagct
      901 cagaagttca gcctggccca ctccatgttg gccatcagtg gtcacctaga cagcgacgat
      961 gatagtggct ccggaagcct ggttggcatt gacaacaaaa tcgagcaagc catggacttg
     1021 gtgaagtccc acctcatgtt tgcggtccgg gaggaggtgg aggtgctgaa ggagcagatc
     1081 cgggaactgg cggagcggaa cgctgcgctg gagcaggaga atgggctgct gcgcgccctg
     1141 gccagcccgg agcagctggc tcagctgccc tcctcggggg tcccacggct tgggccccct
     1201 gcgcccaatg ggccctccgt ctgcccgact ttcttgtaca aagtggttga tatcggtaag
     1261 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1321 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattt agcgagagcc
     1381 cccgcgacac c
//
