LOCUS       sequence-287157-        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3538
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1058..1082)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1090..1131
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cctttcccaa cagctctcct
       61 gctgctaata cttttttagt agattccttg atcagtgcct gcaggagtga cagtttttat
      121 tccagcagcg ccagcatgta catgccacca cctagcgcag acatggggac ctatggaatg
      181 caaacctgtg gactgctccc gtctctggcc aaaagagaag tgaaccacca aaatatgggt
      241 atgaatgtgc atccttatat acctcaagta gacagttgga cagatccgaa cagatcttgt
      301 cgaatagagc aacctgttac acagcaagtc cccacttgct ccttcaccac caacattaag
      361 gaagaatcca attgctgcat gtattctgat aagcgcaaca aactcatttc ggccgaggtc
      421 ccttcgtacc agaggctggt ccctgagtct tgtcccgttg agaaccctga ggttcccgtc
      481 cctggatatt ttagactgag tcagacctac gccaccggga aaacccaaga gtacaataat
      541 agccccgaag gcagctccac tgtcatgctc cagctcaacc ctcgtggcgc ggccaagccg
      601 cagctctccg ctgcccagct gcagatggaa aagaagatga acgagcccgt gagcggccag
      661 gagcccacca aagtctccca ggtggagagc cccgaggcca aaggcggcct tcccgaagag
      721 aggagctgcc tggctgaggt ctccgtgtcc agtcccgaag tgcaggagaa ggaaagcaaa
      781 gaggaaatca agtctgatac accaaccagc aattggctca ctgcaaagag tggcagaaag
      841 aagaggtgcc cttacactaa gcaccaaacg ctggaattag aaaaagagtt cttgttcaat
      901 atgtacctca cccgcgagcg ccgcctagag atcagtaaga gcgttaacct caccgacagg
      961 caggtcaaga tttggtttca aaaccgccga atgaaactca agaagatgag ccgagagaac
     1021 cggatccgag aactgaccgc caacctcacg ttttcttacc caactttctt gtacaaagtg
     1081 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1141 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1201 gacaactcat gcggccacga ttcagc
//
